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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9871

Title: Influence of freeze-thawing on hyaluronic acid binding of human spermatozoa
Authors: Nijs, Martine
Cox, Annemie
Janssen, Mia
Vanheusden, Elke
CASTRO SANCHEZ, Amparo Yovanna
THIJS, Herbert
Issue Date: 2009
Citation: REPRODUCTIVE BIOMEDICINE ONLINE, 19(2). p. 202-206
Abstract: Mature human spermatozoa have at least three specific hyaluronic acid (HA) binding proteins present on their sperm membrane. These receptors play a role in the acrosome reaction, hyaluronidase activity, hyaluronan-mediated motility and sperm-zona and sperm-oolemmal binding. Cryopreservation of spermatozoa can cause ultrastructural and even molecular damage. The aim of this study was to investigate if HA binding receptors of human spermatozoa remain functional after freeze-thawing. Forty patients were enrolled in the study. Semen samples were analysed before and after cryopreservation. Parameters analysed included concentration, motility, morphology and hyaluronan binding. Samples were frozen in CBS straws using a glycerol-glucose-based cryoprotectant. HA binding was studied using the sperm-hyaluronan binding assay. Freeze-thawing resulted in a significant decline in motility: the percentage of motile spermatozoa reduced from 50.6 to 30.3% (P < 0.001). HA binding properties of frozen-thawed spermatozoa remained unchanged after the freeze-thawing process: 68.5 +/- 17.1% spermatozoa of the neat sample were bound to HA, as were 71.3 +/- 20.4 of the frozen-thawed sample. This study indicates that freeze-thawing did not alter the functional hyaluronan binding sites of mature motile spermatozoa, and therefore will not alter their fertilizing potential.
Notes: [Nijs, Martine; Creemers, Eva; Cox, Annemie; Janssen, Mia; Vanheusden, Elke; Ombelet, Willem] Ziekenhuis Oost Limburg, Genk Inst Fertil Technol, Genk, Belgium. [Castro-Sanchez, Yovanna; Thijs, Herbert] Univ Hasselt, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. [Castro-Sanchez, Yovanna; Thijs, Herbert] Katholieke Univ Leuven, Louvain, Belgium.
URI: http://hdl.handle.net/1942/9871
Link to publication: http://www.rbmonline.com/Article/4039
ISI #: 000268786600010
ISSN: 1472-6483
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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