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Title: Identification and comparative analysis of the structural proteomes of phi KZ and EL, two giant Pseudomonas aeruginosa bacteriophages
Authors: Lecoutere, Elke
Ceyssens, Pieter-Jan
Miroshnikov, Konstantin A.
Mesyanzhinov, Vadim V.
Krylov, Victor N.
NOBEN, Jean-Paul
Hertveldt, Kirsten
Volckaert, Guido
Lavigne, Rob
Issue Date: 2009
Citation: PROTEOMICS, 9(11). p. 3215-3219
Abstract: Giant bacteriophages phi KZ and EL of Pseudomonas aeruginosa contain 62 and 64 structural proteins, respectively, identified by ESI-MS/MS on total virion particle proteins. These identifications verify gene predictions and delineate the genomic regions dedicated to phage assembly and capsid formation (30 proteins were identified from a tailless phi KZ mutant). These data form the basis for future structural studies and provide insights into the relatedness of these large phages. The 4 KZ structural proteome strongly correlates to that of Pseudomonas chlororaphis bacteriophage 201 phi 2-1. Phage EL is more distantly related, shown by its 26 non-conserved structural proteins and the presence of genomic inversions.
Notes: [Lecoutere, Elke; Ceyssens, Pieter-Jan; Hertveldt, Kirsten; Volckaert, Guido; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, B-3001 Louvain, Belgium. [Miroshnikov, Konstantin A.; Mesyanzhinov, Vadim V.] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia. [Krylov, Victor N.] State Inst Genet & Select Ind Microorganisms, Moscow, Russia. [Noben, Jean-Paul; Robben, Johan] Univ Hasselt, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul; Robben, Johan] Univ Hasselt, Sch Life Sci, Transnat Univ Limburg, Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/9795
DOI: 10.1002/pmic.200800727
ISI #: 000267414500025
ISSN: 1615-9853
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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