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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9769

Title: Silencing rapsyn in vivo decreases acetylcholine receptors and augments sodium channels and secondary postsynaptic membrane folding
Authors: Martinez-Martinez, Pilar
Phemambucq, Marko
Steinbusch, Laura
Schaeffer, Laurent
Berrih-Aknin, Sonia
Duimel, Hans
Frederik, Peter
Molenaar, Peter C.
Losen, Mario
Issue Date: 2009
Citation: NEUROBIOLOGY OF DISEASE, 35(1). p. 14-23
Abstract: The receptor-associated protein of the synapse (rapsyn) is required for anchoring and stabilizing the nicotinic acetylcholine receptor (AChR) in the postsynaptic membrane of the neuromuscular junction (NMJ) during development. Here we studied the role of rapsyn in the maintenance of the adult NMJ by reducing rapsyn expression levels with short hairpin RNA (shRNA). Silencing rapsyn led to the average reduction of the protein levels of rapsyn (31% loss) and AChR (36% loss) at the NMJ within 2 weeks, corresponding to previously reported half life of these proteins. On the other hand, the sodium channel protein expression was augmented (66%) in rapsyn-silenced muscles. Unexpectedly, at the ultrastructural level a significant increase in the amount of secondary folds of the postsynaptic membrane in silenced muscles was observed. The neuromuscular transmission in rapsyn-silenced muscles was mildly impaired. The results suggest that the adult NMJ can rapidly produce postsynaptic folds to compensate for AChR and rapsyn loss. (C) 2009 Elsevier Inc. All rights reserved.
Notes: [Martinez-Martinez, Pilar; Phemambucq, Marko; Steinbusch, Laura; Molenaar, Peter; De Baets, Marc H.; Losen, Mario] Maastricht Univ, Dept Neurosci, Sch Mental Hlth & Neurosci, NL-6200 MD Maastricht, Netherlands. [Schaeffer, Laurent] Ecole Normale Super Lyon, Equipe Differenciat Neuromusculaire, Inst Federatif Rech 128, Ctr Natl Rech Sci,Unite Mixte Rech 5161, F-69364 Lyon 07, France. [Berrih-Aknin, Sonia] Univ Paris 11, Hop Marie Lannelongue, CNRS, IPSC,UMR 8162, Paris, France. [Duimel, Hans; Frederik, Peter] Maastricht Univ, Dept Pathol, Electron Microscopy Unit, NL-6200 MD Maastricht, Netherlands. [De Baets, Marc H.] Hasselt Univ, Biomed Res Inst BIOMED, Neuroimmunol Grp, Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/9769
DOI: 10.1016/j.nbd.2009.03.008
ISI #: 000267095600003
ISSN: 0969-9961
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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