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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9707

Title: Comparative proteome analysis from Erwinia amylovora grown in vitro and in planta
Authors: THOELEN, Mieke
NOBEN, Jean-Paul
ROBBEN, Johan
VALCKE, Roland
Deckers, T.
Issue Date: 2008
Publisher: INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE
Citation: Johnson, KB. & Stockwell, VO. (Ed.) PROCEEDINGS OF THE ELEVENTH INTERNATIONAL WORKSHOP ON FIRE BLIGHT. p. 179-181.
Series/Report: ACTA HORTICULTURAE
Abstract: Fire blight, caused by the bacterium Erwinia amylovora, affects most species of the Malaceae and some species in other families of the Rosales. The severity of the disease results from its destructive character on the one hand and the lack of effective control methods on the other hand. To gain a better insight into the infection process and proteins involved, the proteome of bacteria grown in vitro and in planta (M9 kloon 29, apple rootstocks) was studied. After two-dimensional electrophoresis, differentially regulated protein spots were excised, trypsinized and analysed by LC-ESI-MS/MS. A total of 127 differentially expressed proteins were identified of which 108 are up-regulated in planta and 19 proteins are down-regulated. Most of the identified proteins ware involved in general metabolism; however, 26% are related to oxidative stress. Numerous defence mechanisms against oxidative stress are strongly up-regulated in bacteria in planta. The protective mechanisms can be divided into three groups: prevention of ROS generation, detoxification of radicals and repair of damaged elements. Our hypothesis is that these protective mechanisms are one of the strategies used by Erwinia amylovora to escape plant defence mechanisms.
Notes: [Thoelen, M.; Noben, J. P.; Robben, J.; Valcke, R.] Hasselt Univ, Dept SBG BIOMED, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/9707
Link to publication: http://www.actahort.org/books/793/793_23.htm
ISI #: 000259569500023
ISBN: 978-90-6605-511-7
ISSN: 0567-7572
Category: C1
Type: Proceedings Paper
Validation: ecoom, 2009
Appears in Collections: Research publications

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