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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9433

Title: Kinetic properties of the alpha(2) homo-oligomeric glycine receptor impairs a proper synaptic functioning
Authors: Mangin, J.A.
Baloul, M.
de Carvalho, L.P.
Rogister, B.
RIGO, Jean-Michel
Legendre, P.
Issue Date: 2003
Citation: JOURNAL OF PHYSIOLOGY-LONDON, 553(2). p. 369-386
Abstract: Ionotropic glycine receptors (GlyRs) are present in the central nervous system well before the establishment of synaptic contacts. Immature nerve cells are known, at least in the spinal cord, to express alpha(2) homomeric GlyRs, the properties of which are relatively unknown compared to those of the adult synaptic form of the GlyR (mainly alpha(1)/beta heteromeres). Here, the kinetics properties of GlyRs at the single-channel level have been recorded in real-time by means of the patch-clamp technique in the outside-out configuration coupled with an ultra-fast flow application system (< 100 μs). Recordings were performed on chinese hamster ovary (CHO) cells stably transfected with the a, GlyR subunit. We show that the onset, the relaxation and the desensitisation of α(2) homomeric GlyR-mediated currents are slower by one or two orders of magnitude compared to synaptic mature GlyRs and to other ligand-gated ionotropic channels involved in fast synaptic transmission. First latency analysis performed on single GlyR channels revealed that their slow activation time course was due to delayed openings. When synaptic release of glycine was mimicked (1 mM glycine; 1 ms pulse duration), the opening probability of α(2) homomeric GlyRs was low (P-o &AP; 0.1) when compared to mature synaptic GlyRs (P-o = 0.9). This low P-o is likely to be a direct consequence of the relatively slow activation kinetics of α(2) homomeric GlyRs when compared to the activation kinetics of mature α(1)/β GlyRs. Such slow kinetics suggest that embryonic α(2) homomeric GlyRs cannot be activated by fast neurotransmitter release at mature synapses but rather could be suited for a non-synaptic paracrine-like release of agonist, which is known to occur in the embryo.
Notes: Univ Paris 06, CNRS, UMR 7102, F-75252 Paris 05, France. Fac Med Liege, Ctr Rech Neurobiol Cellulaire & Mol, B-4020 Liege, Belgium.
URI: http://hdl.handle.net/1942/9433
DOI: 10.1113/jphysiol.2003.052142
ISI #: 000187279700005
ISSN: 0022-3751
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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