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Title: Genome Survey and Characterization of Endophytic Bacteria Exhibiting a Beneficial Effect on Growth and Development of Poplar Trees
Authors: Taghavi, Safiyh
Garafola, Craig
Monchy, Sebastien
Newman, Lee
Hoffman, Adam
BARAC, Tanja
Issue Date: 2009
Abstract: The association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. Our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (Populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. Members of the Gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. As representatives for the dominant genera of endophytic gammaproteobacteria, we selected Enterobacter sp. strain 638, Stenotrophomonas maltophilia R551-3, Pseudomonas putida W619, and Serratia proteamaculans 568 for genome sequencing and analysis of their plant growth-promoting effects, including root development. Derivatives of these endophytes, labeled with gfp, were also used to study the colonization of their poplar hosts. In greenhouse studies, poplar cuttings (Populus deltoides x Populus nigra DN-34) inoculated with Enterobacter sp. strain 638 repeatedly showed the highest increase in biomass production compared to cuttings of noninoculated control plants. Sequence data combined with the analysis of their metabolic properties resulted in the identification of many putative mechanisms, including carbon source utilization, that help these endophytes to thrive within a plant environment and to potentially affect the growth and development of their plant hosts. Understanding the interactions between endophytic bacteria and their host plants should ultimately result in the design of strategies for improved poplar biomass production on marginal soils as a feedstock for biofuels.
Notes: [Taghavi, Safiyh; Garafola, Craig; Monchy, Sebastien; van der Lelie, Daniel] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. [Newman, Lee; Hoffman, Adam] Univ S Carolina, Arnold Sch Publ Hlth, Columbia, SC 29208 USA. [Newman, Lee; Hoffman, Adam] Savannah River Ecol Lab, Aiken, SC 29802 USA. [Weyens, Nele; Barac, Tanja; Vangronsveld, Jaco] Univ Hasselt, Dept Environm Biol, CMK, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/9255
DOI: 10.1128/AEM.02239-08
ISI #: 000262690100025
ISSN: 0099-2240
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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