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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9254

Title: On the use of Z-scan fluorescence correlation experiments on giant unilamellar vesicles
Authors: GIELEN, Ellen
VAN DE VEN, Martin
Margineanu, Anca
Dedecker, Peter
Van der Auweraer, Mark
Engelborghs, Yves
Hofkens, Johan
Issue Date: 2009
Citation: CHEMICAL PHYSICS LETTERS, 469(1-3). p. 110-114
Abstract: Z-scan fluorescence correlation spectroscopy (FCS) uses multiple focus planes to determine diffusion coefficients in supported phospholipid bilayers (SPBs) without the need for extrinsic calibration. The approach can be used to discriminate diffusion modes. In this work, Z-scan FCS was applied to study the diffusion of the fluorescent lipid analogue DiI-C-18(5) in the top membrane of giant unilamellar vesicles (GUVs) in the electroformation chamber. In contrast to SPBs, the intrinsic estimate of the radial waist might differ from the one obtained by extrinsic calibration. We describe a pragmatic approach to verify the validity of the Z-scan approach under these conditions. (C) 2008 Elsevier B.V. All rights reserved.
Notes: [Gielen, Ellen; vandeVen, Martin; Ameloot, Marcel] Hasselt Univ, B-3590 Diepenbeek, Belgium. [Gielen, Ellen; vandeVen, Martin; Ameloot, Marcel] Transnat Univ Limburg, Sch Life Sci, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Gielen, Ellen; Engelborghs, Yves] Katholieke Univ Leuven, Lab Biomol Dynam, B-3001 Heverlee, Belgium. [Margineanu, Anca] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England. [Dedecker, Peter; Van der Auweraer, Mark; Hofkens, Johan] Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium.
URI: http://hdl.handle.net/1942/9254
DOI: 10.1016/j.cplett.2008.12.061
ISI #: 000262801400022
ISSN: 0009-2614
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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