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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/9181

Title: Spinal congenital dermal sinus in a chick embryo model Laboratory investigation
Authors: van Aalst, Jasper
Boselie, Toon F. M.
BEULS, Emile
Vles, Johan S. H.
van Straaten, Henny W. M.
Issue Date: 2009
Publisher: AMER ASSOC NEUROLOGICAL SURGEONS
Citation: JOURNAL OF NEUROSURGERY-PEDIATRICS, 3(1). p. 24-28
Abstract: Object. The origin of spinal congenital dermal sinuses is not known. A local nondisjunction of the closing neural tube and the epidermal ectoderm is thought to be the cause of this malformation. In this experimental study, a nondisjunction was mimicked in chick embryos to create an animal model for the dermal sinus. Methods. A piece of amniotic tissue was implanted in the closing neural tube in ovo in chick embryos at 2 days of incubation. A total of 50 embryos were manipulated. After a further incubation time of 2-7 days, the embryos were macroscopically and histologically evaluated. Results. Dermal sinus-like anomalies were induced in 24 embryos. The induced abnormalities varied from superficial, epidermal lesions to epidermal dimples continuing as a strand of tissue toward the neural tube. This strand invariably was of nonneuronal origin. Additionally, in 3 embryos a split cord malformation was noted, most likely caused by damage to the neural tube during implantation. Conclusions. Implantation of donor amniotic tissue in the closing chick neural tube does result in a dimple, from which a strand of tissue continues to the neural tube in various cases, indicating that formation of a dermal sinus-like anomaly can be successfully induced by experimental continuation of the connection between neural tube and surface ectoderm. This finding strengthens the hypothesis that a human dermal sinus arises after nondisjunction of neural tube and surface ectoderm. (DOI: 10.3171/2008.10.PEDS08184)
Notes: [van Aalst, Jasper; Boselie, Toon F. M.; Beuls, Emile A. M.] Univ Hosp Maastricht, Dept Neurosurg, NL-6202 AZ Maastricht, Netherlands. [Vles, Johan S. H.] Univ Hosp Maastricht, Dept Child Neurol, NL-6202 AZ Maastricht, Netherlands. [van Straaten, Henny W. M.] Univ Maastricht, Dept Anat & Embryol, Maastricht, Netherlands. [Beuls, Emile A. M.] Univ Hasselt, Dept Basic Med Sci, Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/9181
DOI: 10.3171/2008.10.PEDS08184
ISI #: 000262020100005
ISSN: 1933-0707
Category: A1
Type: Journal Contribution
Validation: ecoom, 2010
Appears in Collections: Research publications

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