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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/907

Title: Impedimetric Immunosensors Based on the Conjugated Polymer PPV
Authors: COOREMAN, Peter
THOELEN, Ronald
MANCA, Jean
VAN DE VEN, Martin
VERMEEREN, Veronique
MICHIELS, Luc
AMELOOT, Marcel
WAGNER, Patrick
Issue Date: 2005
Publisher: ELSEVIER ADVANCED TECHNOLOGY
Citation: Biosensors and Bioelectronics, 20(10). p. 2151-2156
Abstract: In the work reported here, we investigated the interaction between the semiconducting polymer MDMO-PPV and antibodies against the fluorescent dyes FITC and Cy5. The antibodies are adsorbed physically onto thin polymer films on gold electrodes, as seen in AFM images of these films. By tuning the antibody concentration, the contact angle of distilled water with the film can be made to vary between 95° and 50°, showing that different surface densities of antibody can be obtained. That these biosensor films specifically bind their antigenic fluorescent molecules from PBS buffer solution is demonstrated by confocal fluorescence microscopy. Specific antigen-antibody recognition is demonstrated by lack of crosssensitivity between the two antibodies and their antigens. In a biosensor prototype based on differential impedance spectroscopy, these polymer films show a clear response to 1 ppb antigen solution, with a time constant of 2 to 3 minutes.
URI: http://hdl.handle.net/1942/907
DOI: 10.1016/j.bios.2004.08.029
ISI #: 000227845700036
ISSN: 0956-5663
Category: A1
Type: Journal Contribution
Validation: ecoom, 2006
Appears in Collections: Research publications

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