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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8565

Title: Rapid, postmortem 9.4 T MRI of spinal cord injury: Correlation with histology and survival times
Authors: Scholtes, Felix
Phan-Ba, Remy
Theunissen, Evi
Brook, Gary
Franzen, Rachelle
Bouhy, Delphine
Gelan, Jan
Martin, Didier
Schoenen, Jean
Issue Date: 2008
Citation: JOURNAL OF NEUROSCIENCE METHODS, 174(2). p. 157-167
Abstract: High field magnetic resonance imaging (MRI) has been increasingly used to assess experimental spinal cord injury (SCI). In the present investigation, after partial spinal cord injury and excision of the whole spine, pathological changes of the spinal cord were studied in spinal cord-spine blocks, from the acute to the chronic state (24 h to 5 months). Using proton density (PD) weighted imaging parameters at a magnetic field strength of 9.4 tesla (T), acquisition times ranging from < 1 to 10 h per specimen were used. High in-plane pixel resolution (68 and 38 mu m, respectively) was obtained, as well as high signal-to-noise ratio (SNR), which is important for optimal contrast settings. The quality of the resulting MR images was demonstrated by comparison with histology. The cord and the lesion were shown in their anatomical Surroundings, detecting cord swelling in the acute phase (24 h to 1 week) and cord atrophy at the chronic stage. Haemorrhage was detected as hypo-intense signal. Oedema, necrosis and scarring were hyper-intense but could not be distinguished. Histology confirmed that the anatomical delimitation of the lesion extent by MRI was precise, both with high and moderate resolution. The present investigation thus demonstrates the precision of spinal cord MRI at different survival delays after compressive Partial SCI and establishes efficient imaging parameters for postmortem PD MRI. (C) 2008 Elsevier B.V. All rights reserved.
Notes: [Scholtes, Felix; Phan-Ba, Remy; Franzen, Rachelle; Bouhy, Delphine; Schoenen, Jean] Univ Liege, Ctr Cellular & Mol Neurobiol CNCM, Dept Neuroanat, B-4000 Liege, Belgium. [Scholtes, Felix; Martin, Didier] CHU, Dept Neurosurg, B-4000 Liege, Belgium. [Adriaensens, Peter; Gelan, Jan] Univ Hasselt, Inst Mat Res IMO, Div Chem, B-3590 Diepenbeek, Belgium. [Theunissen, Evi] Univ Hasselt, Biomed Res Inst BIOMED, B-3590 Diepenbeek, Belgium. [Brook, Gary] Rhein Westfal TH Aachen, Dept Neuropathol, D-52057 Aachen, Germany. [Schoenen, Jean] Citadelle Hosp, Univ Dept Neurol, B-4000 Liege, Belgium.
URI: http://hdl.handle.net/1942/8565
DOI: 10.1016/j.jneumeth.2008.06.038
ISI #: 000259905600001
ISSN: 0165-0270
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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