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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8530

Title: Diffusion of myelin oligodendrocyte glycoprotein in living OLN-93 cells investigated by raster-scanning image correlation spectroscopy (RICS)
Authors: GIELEN, Ellen
VAN DE VEN, Martin
Gijsbers, Rik
Debyser, Zeger
RIGO, Jean-Michel
Hofkens, Johan
Engelborghs, Yves
Issue Date: 2008
Citation: JOURNAL OF FLUORESCENCE, 18(5). p. 813-819
Abstract: Many membrane proteins and lipids are partially confined in substructures ranging from tens of nanometers to micrometers in size. Evidence for heterogeneities in the membrane of oligodendrocytes, i.e. the myelin-producing cells of the central nervous system, is almost exclusively based on detergent methods. However, as application of detergents can alter the membrane phase behaviour, it is important to investigate membrane heterogeneities in living cells. Here, we report on the first investigations of the diffusion behavior of the myelin-specific protein MOG (myelin oligodendrocyte glycoprotein) in OLN-93 as studied by the recently developed RICS (raster-scanning image correlation spectroscopy) technique. We implemented RICS on a standard confocal laser-scanning microscope with one-photon excitation and analog detection. Measurements on FITC-dextran were used to evaluate the performance of the system and the data analysis procedure.
Notes: Hasselt Univ, Biomed Res Inst, Lab Cell Physiol, B-3590 Diepenbeek, Belgium. Catholic Univ Louvain, Lab Biomol Dynam, B-3001 Heverlee, Belgium. Eindhoven Univ Technol, Dept Appl Phys, NL-5600 MB Eindhoven, Netherlands. Catholic Univ Louvain, Lab Mol Virol & Gene Therapy, B-3000 Louvain, Belgium. Catholic Univ Louvain, Lab Photochem & Spect, B-3001 Heverlee, Belgium. Transnatl Univ Limburg, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/8530
DOI: 10.1007/s10895-007-0308-8
ISI #: 000259187600007
ISSN: 1053-0509
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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