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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8470

Title: Impact of luxS and suppressor mutations on the gastrointestinal transit of Lactobacillus rhamnosus GG
Authors: Lebeer, S
Claes, IJJ
Verhoeven, TLA
Shen, C
Ceuppens, JL
Vanderleyden, J
De Keersmaecker, SCJ
Issue Date: 2008
Abstract: It is generally believed that probiotic bacteria need to survive gastrointestinal transit to exert a health-promoting effect. In this study, a genuine luxS mutant and a luxS mutant containing unknown suppressor mutations of the probiotic strain Lactobacillus rhamnosus GG were compared to the wild type for survival and persistence in the murine gastrointestinal tract. The LuxS enzyme, catalyzing the production of the autoinducer-2 signaling molecule, also forms an integral part of the activated methyl cycle and the metabolism of methionine and cysteine. The genuine luxS mutant CMPG5412 showed drastically reduced persistence in mice, which was related to less survival in simulated gastric juice, indicating that LuxS metabolism is crucial for the gastric stress resistance of L. rhamnosus GG. The suppressor mutations in the other luxS mutant, CMPG5413, appear to compensate for the metabolic defects of the luxS mutation and to restore the resistance to gastric juice but cause a defect in adherence, biofilm formation, and exopolysaccharide production. The shorter residence time of this suppressor mutant in the murine gastrointestinal tract indicates a role for biofilm formation and exopolysaccharides in the persistence capacity of L. rhamnosus GG.
Notes: Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Louvain, Belgium. Katholieke Univ Leuven, Univ Hosp, Div Gastroenterol, B-3000 Louvain, Belgium. Univ Hasselt, Biomed Res Inst, Histol Lab, B-3590 Diepenbeek, Belgium. Katholieke Univ Leuven, Univ Hosp, Div Clin Immunol, B-3000 Louvain, Belgium.
URI: http://hdl.handle.net/1942/8470
DOI: 10.1128/AEM.00133-08
ISI #: 000258474400016
ISSN: 0099-2240
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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