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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8377

Title: The intron-containing genome of the lytic Pseudomonas phage LUZ24 resembles the temperate phage PaP3
Authors: Ceyssens, PJ
Hertveldt, K
Ackermann, Hans-W.
NOBEN, Jean-Paul
Demeke, M
Volckaert, G
Lavigne, R
Issue Date: 2008
Publisher: Academic Press
Citation: VIROLOGY, 377(2). p. 233-238
Abstract: The virulent Pseudomonas aeruginosa bacteriophage LUZ24 (45,625 bp) was isolated from hospital sewage. It belongs to the family of the Podoviridae, and carries a bidirectionally transcribed dsDNA genome delineated by two direct terminal repeats of 184 bp. In vitro transcriptional analysis identified seven sigma(70) promoters, revealing a bias towards stronger promoter strength in the late genomic region. Reverse transcription demonstrated in vivo splicing of a 668 bp Group I intron embedded inside the DNA polymerase gene. Using mass spectrometry, nine structural proteins were identified as part of the phage particle. The lytic characteristics of LUZ24 are evaluated against its genomic content, which displays an overall 71% sequence similarity to the temperate phage PaP3.
URI: http://hdl.handle.net/1942/8377
DOI: 10.1016/j.virol.2008.04.038
ISI #: 000257223500002
ISSN: 0042-6822
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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