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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8298

Title: Cytogenesis in the dentate gyrus after neonatal hyperthermia-induced seizures: What becomes of surviving cells?
Authors: Lemmens, EMP
Schijns, OEMG
BEULS, Emile
Hoogland, G
Issue Date: 2008
Citation: EPILEPSIA, 49(5). p. 853-860
Abstract: Purpose: Febrile seizures (FS) are early-life seizures thought to play a role in epileptogenesis. By labeling cells that were dividing immediately following experimental FS, we previously demonstrated that significantly more of these newborn cells in the dentate gyrus (DG) survived 8 weeks later, relative to animals that did not experience FS. The purpose of the present study was to determine the long-term fate of these newborn cells. Methods: On postnatal day (PN) 10, hyperthermia-induced seizures (HT, +/- 42 degrees C core temperature) were evoked in Sprague-Dawley rats and littermates were used as normothermia controls (NT, +/- 35 degrees C core temperature). From PN11 to PN16, rats were injected with bromodeoxyuridine (BrdU) to label dividing cells. At PN66, we evaluated the number of BrdU-labeled cells in the DG that colocalized with the neuronal marker NeuN, glial marker glial fibrillary acidic protein (GFAP), neuronal excitatory amino acid transporter 3 (EAAT3), GABAergic neuronal marker glutamic acid decarboxylase 67 (GAD67) or microglia marker tomato lectin (TL). Results: In all rats, almost all BrdU-labeled cells in the DG, that showed double-labeling, colocalized with NeuN, and rarely with GFAP, GAD67, or TL. In NT controls and HT rats that did not experience seizures ("HT-no seizures"), similar to 23% of BrdU-labeled cells colocalized with EAAT3, which was significantly different from 14% in HT rats that did experience seizures (HT + FS). Discussion: Early-life seizures decrease the population of newborn cells that survive and mature into EAAT3-positive neurons and do not affect the GABAergic cell population. This may affect hippocampal physiology in young adulthood.
Notes: Maastricht Univ, Div Neurosci, Dept Psychiat & Neuropsychol, NL-6200 MD Maastricht, Netherlands. Univ Hosp Maastricht, Dept Neurosurg, Maastricht, Netherlands. European Grad Sch Neurosci EURON Maastricht, Maastricht, Netherlands. Hasselt Univ, Dept Anat & Mat Res, Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/8298
DOI: 10.1111/j.1528-1167.2007.01476.x
ISI #: 000255480500013
ISSN: 0013-9580
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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