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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8274

Title: Variability of polymorphic families of three types of xylanase inhibitors in the wheat grain proteome
Authors: Courtin, CM
CROES, Kristof
Gebruers, K.
NOBEN, Jean-Paul
Samyn, B.
Debyser, G.
Van Beeumen, J.
Delcour, CM
Issue Date: 2008
Citation: PROTEOMICS, 8(8). p. 1692-1705
Abstract: Cereals contain proteinaceous inhibitors of endo-beta-1,4-xylanases (E.C., xylanases). Since these xylanase inhibitors (XIs) are only active against xylanases of microbial origin and do not interact with plant endogenous xylanases, they are believed to act as a defensive barrier against phytopathogenic attack. So far, three types of XIs have been identified, i.e. Triticum aestivum XI (TAXI), xylanase inhibiting protein (XIP), and thaumatin-like XI (TIXI) proteins. In this study the variation in XI forms present in wheat grain was elucidated using high-resolution 2-DE in combination with LC-ESI-MS/MS and biochemical techniques. Reproducible 2-DE fingerprints of TAXI-, XIP-, and TIXI-type XIs, selectively purified from whole meal of three European wheat cultivars using cation exchange chromatography followed by affinity chromatography, were obtained using a pH-gradient of 6 to 11 and a molecular mass range of 10 to 60 kDa. Large polymorphic XI families, not known to date, which exhibit different pl-and/or molecular mass values, were visualised by colloidal CBB staining. Identification of distinct genetic variants by MS/MS-analysis provides a partial explanation for the observed XI heterogeneity. Besides genetic diversity, PTMs, such as glycosylation, account for the additional complexity of the 2-DE patterns.
Notes: Katholieke Univ Leuven, Dept Microbial & Mol Syst, Lab Food Chem & Biochem, B-3001 Louvain, Belgium. Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. Univ Ghent, Lab Protein Biochem & Protein Engn, B-9000 Ghent, Belgium.
URI: http://hdl.handle.net/1942/8274
DOI: 10.1002/pmic.200700813
ISI #: 000255314300014
ISSN: 1615-9853
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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