Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8264

Title: Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations
Authors: REMANS, Tony
Mathijsen, Dennis
Issue Date: 2008
Publisher: SPRINGER
Citation: PLANTA, 227(6). p. 1343-1349
Abstract: Accurate quantification by real-time RT-PCR relies on normalisation of the measured gene expression data. Normalisation with multiple reference genes is becoming the standard, but the best reference genes for gene expression studies within one organism may depend on the applied treatments or the organs and tissues studied. Ideally, reference genes should be evaluated in all experimental systems. A number of candidate reference genes for Arabidopsis have been proposed, which can be used as a starting point to evaluate their expression stability in individual experimental systems by available computer algorithms like geNorm and NormFinder. Using this approach, we identified the best three reference genes from a set of ten candidates, which included three traditional "housekeeping" genes, for normalisation of gene expression when roots and leaves of Arabidopsis thaliana are exposed to cadmium (Cd) and copper (Cu). The expression stabilities of AT5G15710 (F-box protein), AT2G28390 (SAND family protein) and AT5G08290 (mitosis protein YLS8) were the highest when considering the effect to the roots and shoots of Cd and Cu treatments. Even though the effect of Cd and excess Cu on the plants is very different, the same best reference genes were identified when considering Cd or Cu treatments separately. This suggests that these three genes may also be suitable when studying the gene expression after exposure of Arabidopsis thaliana to increased concentrations of other metals.
Notes: Hasselt Univ, B-3590 Diepenbeek, Belgium. Remans, T, Hasselt Univ, Agoralaan D, B-3590 Diepenbeek, Belgium. tony.remans@uhasselt.be
URI: http://hdl.handle.net/1942/8264
DOI: 10.1007/s00425-008-0706-4
ISI #: 000254965100015
ISSN: 0032-0935
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
Preprint78.32 kBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.