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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8231

Title: Leukemia inhibitory factor induces an antiapoptotic response in oligodendrocytes through Akt-phosphorylation and up-regulation of 14-3-3
Authors: Slaets, Leen
DUMONT, Debora
VANDERLOCHT, Joris
NOBEN, Jean-Paul
Leprince, P.
ROBBEN, Johan
HENDRIKS, Jerome
STINISSEN, Piet
HELLINGS, Niels
Issue Date: 2008
Publisher: WILEY-VCH VERLAG GMBH
Citation: PROTEOMICS, 8(6). p. 1237-1247
Abstract: Leukemia inhibitory factor (LIF) promotes the survival of oligodendrocytes (OLG) both in vitro and in an animal model of multiple sclerosis. Here, we show that LIF protects mature rat OLG cultures selectively against the combined insult of the proinflammatory cytokines interferon-gamma and tumor necrosis factor-alpha, but it does not protect against oxidative stress nor against staurosporine induced apoptosis. We further demonstrate that LIF activates the janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) and the phosphatidylinositol 3 kinase/Akt pathway in mature OLG. We show that LIF protection is independent of suppressors of cytokine signaling and Bcl-2 mRNA expression levels. To gain further insight into the protective mechanism, a quantitative proteomic approach (DIGE) was applied to identify differentially expressed proteins in LIF-treated OLG. Our results indicate that LIF induces a shift in the cellular machinery toward a prosurvival. execution program, illustrated by an enhanced expression of isoforms of the antiapoptotic molecule 14-3-3. These data provide further insight into the mechanisms of LIF-mediated protection of mature OLGs.
Notes: Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. Univ Liege, Ctr Cellular & Mol Neurobiol, Liege, Belgium.Hellings, N, Hasselt Univ, Biomed Res Inst, Agoralaan Bldg A, B-3590 Diepenbeek, Belgium.niels.hellings@uhasselt.be
URI: http://hdl.handle.net/1942/8231
DOI: 10.1002/pmic.200700641
ISI #: 000254483000014
ISSN: 1615-9853
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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