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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8008

Title: Na-P-i cotransporter type I activity causes a transient intracellular alkalinization during ATP depletion in rabbit medullary thick ascending limb cells
Authors: JANS, Frank
AMELOOT, Marcel
WOUTERS, P.
STEELS, Paul
Issue Date: 2008
Publisher: NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
Citation: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 86(1-2). p. 36-45
Abstract: The cellular pathophysiology of renal ischemia-reperfusion injury was investigated in primary cell cultures from rabbit medullary thick ascending limb (MTAL). Metabolic inhibition (MI) was achieved with cyanide and 2-deoxy-glucose. Sixty minutes of MI caused a profound but reversible decrease in intracellular concentration of ATP ([ATP](i)). Intracellular pH (pH(i)) first decreased after initiation of MI, followed by a transient alkalinization. When [ATP](i) reached its lowest value (<1% of control), the cells slowly acidified to reach a stable pH(i) of 6.92 after 50 min of MI. In the presence of EIPA (10 mu mol/L), the pattern of changes in pH(i) was unchanged and acidification was not increased, indicating that the Na+/H+ exchangers were inactive during ATP depletion. When inorganic phosphate (P-i) or Na+ was omitted from the apical solutions during MI, the transient alkalinization was no longer observed and the cytosol slowly acidified. Experiments on Na+-dependent alkalinizations revealed the presence of a Na-P-i cotransporter in the apical cell membrane. With indirect immunofluorescence, the Na-P-i cotransporter expressed in these primary cell cultures could be identified as Na-P-i type I. Although the exact physiological role of Na-P-i type I still is unresolved, these experiments demonstrate that apical Na-P-i type I activity is increased at the onset of ATP depletion in MTAL cells.
Notes: Univ Hasselt, Dept Physiol, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Catholic Univ Louvain, Dept Anaesthesiol, Univ Hosp Gasthuisberg, B-3000 Louvain, Belgium.Jans, F, Univ Hasselt, Dept Physiol, Biomed Res Inst, B-3590 Diepenbeek, Belgium.frank.jans@hotmaii.com
URI: http://hdl.handle.net/1942/8008
DOI: 10.1139/Y07-114
ISI #: 000253544900006
ISSN: 0008-4212
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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