Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/8001

Title: Dehydroascorbate uptake is impaired in the early response of Arabidopsis plant cell cultures to cadmium
Authors: Horemans, Nele
Raeymaekers, Tine
Van Beek, Kim
Nowocin, Anna
Blust, Ronny
Broos, Katleen
Cuypers, Ann
Vangronsveld, Jaco
Guisez, Yves
Issue Date: 2007
Citation: JOURNAL OF EXPERIMENTAL BOTANY, 58(15-16). p. 4307-4317
Abstract: The balance between antioxidants, such as ascorbate (ASC) and glutathione, and oxidative reactive oxygen species (ROS) is known to play a pivotal role in the response of plant cells to abiotic stress. Here cell cultures of Arabidopsis thaliana were investigated with regard to their response to elevated levels of cadmium. At concentrations <100 mu M, Cd induces a rapid and concentration-dependent H2O2 accumulation. This response could be inhibited by diphenylene iodonium (DPI, 20 mu M). Reverse transcription-PCR analysis of three RBOH (respiratory burst oxidase homologues) genes showed an increased transcription of RBOHF after 15 min. No change in ASC concentration was observed during the first 3 h after Cd addition. In contrast, glutathione levels completely diminished within 1 h. This drop could be attributed to an increase in phytochelatin 4. At the plasma membrane, Cd further induced a significant decrease in dehydroascorbate (DHA) uptake activity (up to 90% inhibition after 4 h). This decrease is not present when cells are treated with LaCl3 before exposure to CdCl2. LaCl3 is a typical inhibitor of Ca channels and prevents Cd uptake in these cells as well as the Cd-induced ROS production. Therefore, these results appear to indicate that Cd uptake is a prerequisite for the change in DHA transport activity. However, DPI did not prevent the drop in DHA uptake activity present in Cd-treated Arabidopsis cells, indicating that this response seems to be independent of the Cd-induced H2O2 production.
Notes: Univ Antwerp, Dept Biol Plant Physiol, B-2020 Antwerp, Belgium. Univ Antwerp, Dept Biol Ecophysiol Biochem & Toxicol, B-2020 Antwerp, Belgium. Hasselt Univ, Ctr Environm Sci Environm Biol, B-3590 Diepenbeek, Belgium.Horemans, N, Univ Antwerp, Dept Biol Plant Physiol, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.nele.horemans@ua.ac.be
URI: http://hdl.handle.net/1942/8001
DOI: 10.1093/jxb/erm291
ISI #: 000253095900026
ISSN: 0022-0957
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
Published version395.85 kBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.