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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/7995

Title: The genome and structural Proteome of YuA, a new Pseudomonas aeruginosa phage resembling M6
Authors: Ceyssens, Pieter-Jan
Mesyanzhinov, Vadim V.
Sykilinda, Nina
Briers, Yves
Roucourt, Bart
Lavigne, Rob
Domashin, Artem
Miroshnikov, Konstantin A.
Volckaert, Guido
Hertveldt, Kirsten
Issue Date: 2008
Citation: JOURNAL OF BACTERIOLOGY, 190(4). p. 1429-1435
Abstract: Pseudomonas aeruginosa phage YuA (Siphoviridae) was isolated from a pond near Moscow, Russia. It has an elongated head, encapsulating a circularly permuted genome of 58,663 bp, and a flexible, noncontractile tail, which is terminally and subterminally decorated with short fibers. The YuA genome is neither Mu- nor lambda-like and encodes 78 gene products that cluster in three major regions involved in (i) DNA metabolism and replication, (ii) host interaction, and (iii) phage particle formation and host lysis. At the protein level, YuA displays significant homology with phages M6, phi JL001, 73, B3, DMS3, and D3112. Eighteen YuA proteins were identified as part of the phage particle by mass spectrometry analysis. Five different bacteria] promoters were experimentally identified using a promoter trap assay, three of which have a sigma(54)-specific binding site and regulate transcription in the genome region involved in phage particle formation and host lysis. The dependency of these promoters on the host sigma(54) factor was confirmed by analysis of an rpoN mutant strain of P. aeruginosa PAO1. At the DNA level, YuA is 91% identical to the recently (July 2007) annotated phage M6 of the Lindberg typing set. Despite this level of DNA homology throughout the genome, both phages combined have 15 unique genes that do not occur in the other phage. The genome organization of both phages differs substantially from those of the other known Pseudomonas-infecting Siphoviridae, delineating them as a distinct genus within this family.
Notes: Katholieke Univ Leuven, Div Gen Technol, B-3001 Louvain, Belgium. Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia. Univ Hasselt, Sch Life Sci, B-3590 Diepenbeek, Belgium. Limburgs Univ Ctr, Biomed Res Inst, Diepenbeek, Belgium.Hertveldt, K, Katholieke Univ Leuven, Div Gen Technol, Kasteelpk Arenberg 21, B-3001 Louvain, Belgium.kirsten.hertveldt@biw.kuleuven.be
URI: http://hdl.handle.net/1942/7995
DOI: 10.1128/JB.01441-07
ISI #: 000253005800032
ISSN: 0021-9193
Category: A1
Type: Journal Contribution
Validation: ecoom, 2009
Appears in Collections: Research publications

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