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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/6658

Title: Cytokin-induced cell death in human oligodendroglial cell lines: 1: synergistic effects of IFN-gamma and TNF-alpha on apoptosis
Authors: BUNTINX, Mieke
MOREELS, Marjan
VANDENABEELE, Frank
LAMBRICHTS, Ivo
RAUS, Jef
STEELS, Paul
STINISSEN, Piet
AMELOOT, Marcel
Issue Date: 2004
Publisher: WILEY-V C H VERLAG GMBH
Citation: Journal of neuroscience research, 76(6). p. 834-845
Abstract: Multiple sclerosis is a chronic inflammatory disease of the central nervous system.Myolin and oligodendrocytes are considered the major targets of injury caused by a cell-mediated immune response. There is circumstantial evidence that proinflammatory cytokines like tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ) could have disease-promoting roles in multiple sclerosis (MS). In the present study,the cytotoxic effects of IFN-γ and TNF-α on the human oligodendroglial cell lines human oligodendroglioma (HOG) and MO3.13 were analyzed. When the oligodendroglial cell lines were cultured in the presence of IFN-γ or TNF-α, apoptotic cell death was observed in both cell lines after >24 hr incubation. Apoptosis was evidenced by a decrease in cell viability, apoptotic changes in cell and nucleus morphology, and disruption of the membrane asymmetry. Our data show that TNF-α and IFN-γ induce apoptosis in a dose-dependent fashion in both oligodendroglial cell lines and that their synergistic effect results in enhanced cell death. Understanding the regulation of cell death pathways in oligodendrocytes is critical for protecting myelin-producing cells and their associated axons during injury in patients with MS.
URI: http://hdl.handle.net/1942/6658
DOI: 10.1002/jnr.20118
Category: A2
Type: Journal Contribution
Appears in Collections: Research publications

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