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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/6332

Title: The effect of recombinant heavy metal resistant endophytic bacteria in heavy metal uptake by their host plant
Authors: Lodewyckx, Cindy
Taghavi, Safieh
Mergeay, Max
Vangronsveld, Jaco
Clijsters, Herman
Van Der Lelie, Daniël
Issue Date: 2001
Citation: International journal of phytoremediation, 3(2). p. 173-187
Abstract: The ncc-nre nickel resistance system of Ralstonia metallidurans 31A was efficiently expressed in Burkholderia cepacia L.S.2.4 and Herbaspirillum seropedicae LMG2284. The heterologous expression of ncc-nre encoded nickel resistance was accompanied by nickel removal from the culture medium. B. cepacia L.S.2.4:: ncc-nre and H. seropedicae LMG2284::ncc-nre were able to remove 35 and 15% nickel, respectively. The capacity to remove nickel through sequestration or bio-precipitation processes and consequently lowering the free nickel concentration could offer interesting benefits for these endophytic bacteria and their host plants. Once inoculated in their host plant, they could possibly alter the nickel speciation and therefore decrease the free ions and thus toxic concentration for the plant metabolism. Lupinus luteus L, when grown on a nickel enriched substrate and inoculated with B. cepacia L.S.2.4::ncc-nre, showed a significant increase (30%) of nickel concentration in the roots, whereas the nickel concentration in the shoots remained comparable with that of the control plants. The inoculation of Lolium perenne (cv Atlas) with the nickel resistance derivative of H. seropedicae LMG2284::ncc-nre resulted in a significant decrease of the nickel concentration in the roots (11%) as well as in the shoots (14%). As this phenomenon was also observed in the Lolium perenne plants inoculated with the wild-type strain LMG2284, the nickel resistance characteristics probably are not responsible for the altered nickel uptake observed.
URI: http://hdl.handle.net/1942/6332
DOI: 10.1080/15226510108500055
ISSN: 1522-6514
Category: A1
Type: Journal Contribution
Validation: vabb, 2010
Appears in Collections: Research publications

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