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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/6256

Title: Effect of cytokinins on the expression of LHCP-genes
Authors: Bloemen, J.
QUANTEN, Lieve
VALCKE, Roland
Issue Date: 2002
Citation: Proceedings PS2001: 12th International Congress on Photosynthesis, Brisbane, Australia, 18-23 August 2001.
Abstract: The light-harvesting chlorophyll a/b-binding proteins (LHCP) function as carrier- proteins of antenna-pigments and are located in the thylakoïd membrane of chloroplasts. They consist of a large family of polypeptides. To get a better insight in the regulation of their genes, we use transgenic tobacco plants with an elevated cytokinin- level. Using a special construct (Pssu-ipt) with a light-inducible promotor (from Pisum sativum), the concentration of cytokinins in the plants can be changed. Accumulation of cytokinins affects the photosynthetic apparatus (Synkova, H. et al., 1999 J. Plant Physiol. 155 173-182). More specific, the effect of cytokinins on the expression of the nuclear-encoded Lhcb-genes will be examined. Plants (Nicotiana tabacum L. cv. Petit Havana SR1) are cultivated on grodan in a greenhouse. This is done for WT as well as for the transgenic plants. Additional illumination is provided 16h a day with Agroson T (400W) and HTQ (400W) lamps (200µmol quanta m-2s-1). Samples are analysed by 2D-electrophoresis and Western blotting.
URI: http://hdl.handle.net/1942/6256
Category: C2
Type: Proceedings Paper
Appears in Collections: Research publications

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