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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4118

Title: Subcellular localization of cadmium in roots and leaves of Arabidopsis thaliana
Authors: VAN BELLEGHEM, Frank
SEMANE, Brahim
VALCKE, Roland
Issue Date: 2007
Citation: NEW PHYTOLOGIST, 173(3). p. 495-508
Abstract: We examined the subcellular cadmium (Cd) localization in roots and leaves of wild-type Arabidopsis thaliana (ecotype Columbia) exposed to environmentally relevant Cd concentrations. Energy-dispersive X-ray microanalysis (EDXMA) was performed on high-pressure frozen and freeze-substituted tissues. In the root cortex, Cd was associated with phosphorus (Cd/P) in the apoplast and sulfur (Cd/S) in the symplast, suggesting phosphate and phytochelatin sequestration, respectively. In the endodermis, sequestration of Cd/S was present as fine granular deposits in the vacuole and as large granular deposits in the cytoplasm. In the central cylinder, symplastic accumulation followed a distinct pattern illustrating the importance of passage cells for the uptake of Cd. In the apoplast, a shift of Cd/S granular deposits from the middle lamella towards the plasmalemma was observed. Large amounts of precipitated Cd in the phloem suggest retranslocation from the shoot. In leaves, Cd was detected in tracheids but not in the mesophyll tissue. Extensive symplastic and apoplastic sequestration in the root parenchyma combined with retranslocation via the phloem confirms the excluder strategy of Arabidopsis thaliana.
Notes: Hasselt Univ, IMOMEC, IMO, B-3590 Diepenbeek, Belgium.Van Belleghem, F, Hasselt Univ, IMOMEC, IMO, Campus Diepenbeek,Agoralaan Bldg D, B-3590 Diepenbeek, Belgium.frank.vanbelleghem@uhasselt.be
URI: http://hdl.handle.net/1942/4118
DOI: 10.1111/j.1469-8137.2006.01940.x
ISI #: 000243478900007
ISSN: 0028-646X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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