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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4090

Title: Proteomic analysis of core breakdown disorder in Conference pears (Pyrus communis L.)
Authors: Pedreschi, Romina
Vanstreels, E.
Carpentier, Sebastien
Hertog, Maarten
Lammertyn, Jeroen
NOBEN, Jean-Paul
Swennen, Rony
Vanderleyden, Jozef
Nicolaie, Bart M.
Issue Date: 2007
Citation: PROTEOMICS, 7(12). p. 2083-2099
Abstract: 2-DE was applied to study core breakdown disorder in controlled atmosphere stored 'Conference' pears. This physiological disorder is characterized by internal browning of the fruit tissue and the development of cavities. Suitable protein phenol extraction/ammonium acetate-methanol precipitation and 2-DE protocols for a wide pH range were established for pear tissue. The protein expression profiles of healthy, sound (intact tissue of pears with core breakdown) and brown-tissue were analyzed with the univariate non-parametric Kolmogorov-Smimov test and multivariate statistical techniques such as principal component analysis and partial least square discriminant analysis. Both statistical approaches revealed interesting differentially expressed proteins between healthy and disordered pears. LC-ESI-MS/MS identification of differentially expressed proteins between healthy and sound tissue revealed their participation in the energy metabolism, the antioxidant system and ethylene biosynthesis. Up-regulated characteristic proteins in brown tissue were mainly involved in energy metabolism and defense mechanisms. Proteomics coupled to univariate and multivariate statistical techniques seems to be an efficient approach to get a better insight into the different mechanisms and pathways leading to the core breakdown disorder.
Notes: Katholieke Univ Leuven, BIOSYST MeBioS Div, B-3001 Heverlee, Belgium. Katholieke Univ Leuven, Div Crop Biotechn, B-3001 Heverlee, Belgium. Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium. Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium.Pedreschi, R, Katholieke Univ Leuven, BIOSYST MeBioS Div, Willem Croylaan 42, B-3001 Heverlee, Belgium.Romina.PedreschiPlasencia@biw.kuleuven.be
URI: http://hdl.handle.net/1942/4090
Link to publication: http://doi.wiley.com/10.1002/pmic.200600723
ISI #: 000247642100013
ISSN: 1615-9853
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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