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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4085

Title: Effects of tetracycline on wild-type and inducible P35So IPT-5/TETR transgenic tobacco plants
Authors: QUANTEN, Lieve
Chaerle, Laury
NOBEN, Jean-Paul
Van Onckelen, Harry
Prinsen, Els
Van der Straeten, Dominique
VALCKE, Roland
Issue Date: 2007
Citation: PHYSIOLOGIA PLANTARUM, 130(2). p. 290-300
Abstract: Introduction of the Agrobacterium ipt gene, coding for isopentenyl transferase, under control of a tetracycline (Tc)-inducible promoter results in a very specific system in which cytokinin levels can be changed. Because Tc belongs to the group of antibiotics that affect 70S ribosomes, it is important to study the effects of Tc on untransformed plants. Although 1 mg l(-1) Tc was previously reported to have no physiological effects, this study revealed several changes in hydroponically grown wild-type Nicotiana tabacum L. cv. Wisconsin. Therefore, lower Tc concentrations (0.1 and 0.2 mg l(-1) Tc) were used to induce ipt-transgenic (tr) plants. Upon induction, real-time PCR analysis showed that the ipt gene was expressed several times higher in roots of tr plants, but not in leaves. Consequently, cytokinin levels were also elevated to a large extent in roots. This resulted in a disturbance of the cytokinin to auxin ratio, leading to an obstructed root growth. In leaves, no significant increase in cytokinins was observed. However, phenotypic and physiological effects, which could be attributed to cytokinin, were apparent in leaves of ipt-induced trs: chlorophyll and carotenoid content were elevated and grana stacking increased. Our study demonstrates that caution has to be taken to determine the 'safe' concentration of inducers when using inducible gene-expression systems.
Notes: Hasselt Univ, Ctr Environm Sci, Dept SBG, Lab Mol & Phys Plant Physiol, B-3590 Diepenbeek, Belgium. Univ Ghent, Dept Mol Genet, Unit Plant Hormone Signalling & Bioimaging, B-9000 Ghent, Belgium. Hasselt Univ, Biomed Onderzoekinst, Immunol Lab, B-3590 Diepenbeek, Belgium. Univ Antwerp, Lab Plant Biochem & Physiol, B-2020 Antwerp, Belgium.VALCKE, R, Hasselt Univ, Ctr Environm Sci, Dept SBG, Lab Mol & Phys Plant Physiol, Bldg D, B-3590 Diepenbeek, Belgium.roland.valcke@uhasselt.be
URI: http://hdl.handle.net/1942/4085
DOI: 10.1111/j.1399-3054.2007.00888.x
ISI #: 000246367500012
ISSN: 0031-9317
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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