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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4075

Title: Neuropeptide imaging on an LTQ with vMALDI source: The complete 'all-in-one' peptidome analysis
Authors: VERHAERT, Peter
Conaway, Maria C. Prieto
Pekar, Tonya M.
Miller, Ken
Issue Date: 2007
Abstract: Direct tissue imaging was performed on dissected insect tissue using a MALDI ion trap to visualize endogenous neuropeptides. Coupling tissue imaging to tandem MSn allows for the identification of previously known species and the ability to identify new ones by de novo sequencing, as searchable databases for insects are sparse. Direct tissue imaging is an attractive technique for the study of neuropeptides as minimal sample preparation is required prior to mass spectrometry. We successfully identified neuropeptides present in the corpora cardiaca and allata of Acheta domesticus (the house cricket). Diagnostic fragments at low m/z were used to distinguish between lipids and neuropeptides. The distribution of peptides appears to be more differentially localized than that of phospholipids, which seem to be more evenly distributed within the tissue. (C) 2006 Elsevier B.V. All rights reserved.
Notes: Delft Univ Technol, Dept Biotechnol, Lab Analyt Biotechnol, NL-2628 BC Delft, Netherlands. Univ Hasselt, Biomed Res Ctr, Hasselt, Belgium. Univ Liege, Mass Spectrometry Lab, B-4000 Liege, Belgium. Flemish Interuniv Inst Biotechnol, Mol Cell Biol Lab, Louvain, Belgium. Thermo Electron Corp, San Jose, CA USA.VERHAERT, PD, Delft Univ Technol, Dept Biotechnol, Lab Analyt Biotechnol, Julianalaan 67, NL-2628 BC Delft, Netherlands.p.d.e.m.verhaert@tudelft.nl
URI: http://hdl.handle.net/1942/4075
DOI: 10.1016/j.ijms.2006.11.008
ISI #: 000243743500012
ISSN: 1387-3806
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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