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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4071

Title: Arginine deficiency in preconfluent intestinal Caco-2 cells modulates expression of proteins involved in proliferation, apoptosis, and heat shock response
Authors: Lenaerts, Kaatje
Bouwman, Freek G.
NOBEN, Jean-Paul
Smit, Egbert
Mariman, Edwin C.
Issue Date: 2007
Citation: PROTEOMICS, 7(4). p. 565-577
Abstract: Arginine is classified as a conditionally essential amino acid required exogenously during catabolic disease states and periods of rapid growth, both characterized by increased arginine utilization. Arginine plays an important role in the intestine, where it is extensively metabolized, and enhances its immune-supportive function and mucosal repair. Cell proliferation is important for the latter process. This study aimed for a better molecular insight in the response to arginine deprivation/supplementation of preconfluent and 5-day-confluent, differentiated Caco-2 intestinal cells. The potential of citrulline to counteract the effects of arginine deprivation was investigated in preconfluent cells. 2-DE combined with MALDI-TOF-MS and the antibody microarray technology were applied. Evidence is provided that arginine deficiency modulates the protein expression profiles of preconfluent Caco-2 cells differently than that of postconfluent differentiated cells. In preconfluent cells, certain proteins changed in direct response to arginine deficiency, whereas other proteins did not, but instead responded during the recovery phase after an arginine/citrulline resupplementation. The protein changes suggest that arginine deprivation decreases cell proliferation and heat shock protein expression, and enhances the cells susceptibility to apoptosis. These processes are critical for proper cell function, and hence a state of arginine deficiency can be detrimental for intestinal cells which proliferate actively in vivo.
Notes: Maastricht Univ, Dept Human Biol, NUTRIM, Maastricht Proteom Ctr, NL-6200 MD Maastricht, Netherlands. Hasselt Univ, Sch Life Sci, Biomed Res Inst, Diepenbeek, Belgium. Hasselt Univ, Sch Life Sci, Transnatl Univ Limburg, Diepenbeek, Belgium.Lenaerts, K, Maastricht Univ, Dept Human Biol, NUTRIM, Maastricht Proteom Ctr, POB 616, NL-6200 MD Maastricht, Netherlands.K.Lenaerts@HB.unimaas.nl
URI: http://hdl.handle.net/1942/4071
DOI: 10.1002/pmic.200600715
ISI #: 000244807500010
ISSN: 1615-9853
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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