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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4070

Title: Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry
Authors: DUMONT, Debora
Ceuppens, Ruben
Van Brussel, Leen
DANIELS, Ruth
NOBEN, Jean-Paul
Van de Plas, Babs
VERHAERT, Peter
Van der Gucht, Estel
Clerens, Stefan
Arckens, Lutgarde
ROBBEN, Johan
Issue Date: 2007
Publisher: ELSEVIER SCIENCE BV
Citation: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 260(2-3). p. 185-194
Abstract: One of the newly developed imaging mass spectrometry (IMS) technologies utilizes matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to map proteins in thin tissue sections. In this study, we evaluated the power of MALDI IMS as we developed it in our (Bruker) MALDI TOF (Reflex IV) and TOF-TOF (Ultraflex II) systems to study myelin patterns in the mouse central nervous system under normal and pathological conditions. MALDI IMS was applied to assess myelin basic protein (MBP) isoform-specific profiles in different regions throughout the mouse brain. The distribution of ions of m/z 14,144 and 18,447 displayed a striking resemblance with white matter histology and were identified as MBP isoform 8 and 5, respectively. In addition, we demonstrated a significant reduction of the MBP-8 peak intensity upon MALDI IMS analysis of focal ethidium bromide-induced demyelinated brain areas. Our MS images were validated by immunohistochemistry using MBP antibodies. This study underscores the potential of MALDI IMS to study the contribution of MBP to demyelinating diseases. (C) 2006 Elsevier B.V. All rights reserved.
Notes: Katholieke Univ Leuven, Lab Neuroplast & Neuroproteom, B-3000 Louvain, Belgium. Hasselt Univ, Biomed Res Inst Biomed & Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. Delft Univ Technol, Lab Analyt Biotechnol, Delft, Netherlands.Arckens, L, Katholieke Univ Leuven, Lab Neuroplast & Neuroproteom, B-3000 Louvain, Belgium.ruben.ceuppens@bio.kuleuven.be debora.dumont@uhasselt.be leen.vanbrussel@bio.kuleuven.be babs.vandeplas@bio.kuleuven.be ruth.daniels@uhasselt.be jeanpaul.noben@uhasselt.be peter.verhaert@udelft.nl estel.vandergucht@bio.kuleuven.be johan.robben@uhasselt.be stefan.clerens@canesis.com Lut.arckens@bio.kuleuven.be
URI: http://hdl.handle.net/1942/4070
DOI: 10.1016/j.ijms.2006.09.007
ISI #: 000243743500013
ISSN: 1387-3806
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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