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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4069

Title: Exploring cDNA phage display for autoantibody profiling in the serum of multiple sclerosis patients - Optimization of the selection procedure
Authors: GOVARTS, Cindy
SOMERS, Klaartje
Hupperts, R.
STINISSEN, Piet
SOMERS, Veerle
Issue Date: 2007
Publisher: BLACKWELL PUBLISHING
Citation: AUTOIMMUNITY, PART A: BASIC PRINCIPLES AND NEW DIAGNOSTIC TOOLS, 1109. p. 372-384
Abstract: We applied a cDNA phage display method called serological antigen selection (SAS) to identify immunogenic targets that evoke an autoantibody response in the serum of multiple sclerosis (MS) patients. This method involves the display of a cDNA expression library, in this study a MS brain library, on filamentous phage and subsequent selection using patient immunoglobulin G (IgG). To apply the SAS technology for autoantibodies in the serum of MS patients, an optimization was necessary to deplete cDNA products that encode IgG fragments derived from B cells present in the MS brain plaques. We describe a differential screening procedure in which positive selection rounds on MS serum and negative selection rounds on healthy control serum were alternated to optimize the selection procedure. As a result, a substantial decrease of IgG-displaying phage clones was observed after each negative selection round, thereby preventing an overgrowth of IgG-displaying phage clones. Our depletion strategy was therefore successful in preventing the enrichment of IgG-displaying phage clones. This approach will facilitate the identification of possible MS-related antigens.
Notes: Hasselt Univ, Inst Biomed Res, B-3590 Diepenbeek, Belgium. Transat Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. Acad Hosp Maastricht, Dept Neurol, Maastricht, Netherlands.SOMERS, V, Hasselt Univ, Inst Biomed Res, Agoralaan Bldg A, B-3590 Diepenbeek, Belgium.veerle.somers@uhasselt.be
URI: http://hdl.handle.net/1942/4069
DOI: 10.1196/annals.1398.043
ISI #: 000249589100044
ISSN: 0077-8923
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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