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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4068

Title: Low cadmium exposure triggers a biphasic oxidative stress response in mice kidneys
Authors: THIJSSEN, Sandy
Horemans, Nele
Issue Date: 2007
Citation: TOXICOLOGY, 236(1-2). p. 29-41
Abstract: Oxidative stress is believed to participate in the early processes of cadmium (Cd)-induced proximal tubular kidney damage. Mice were chronically exposed up to 23 weeks to low Cd concentrations (10 and 100 mg CdCl2/l) via the drinking water. Pro- and antioxidant gene expression levels, glutathione, ascorbate and lipid peroxidation levels were measured. Our study provided evidence for an early and a late stress response in the kidney. Metallothioneins were upregulated from 1 week of exposure on and they stayed important during the whole exposure period. After 8 weeks the expression of Bcl2 (anti -apoptotic), Prdx2 and cytosolic superoxide dismutase (Sod1) was reduced in the group exposed to 100 mg CdCl2/1, which might indicate a response to Cd-stress. However glutathione, ascorbate and lipid peroxidation levels did not significantly change, and the overall redox balance remained stable. Stable Sod2 transcriptional levels suggested that an increased formation of superoxide anions, which can arise upon Cd-induced mitochondrial free radical generation, was not appearing. A second defence activation was observed after 23 weeks: i.e. an increase of catalase (Cat), glutathione peroxidase 4 (Gpx4) and heme oxygenase 1 (Hmox1), together with NADPH oxidase 4 (Nox4), of which the role has not been studied yet in Cd nephrotoxicity. These findings were in contrast with previous studies, where Cd-induced oxidative stress was detrimental when high Cd concentrations were applied. In conclusion our study provided evidence that a chronic exposure to low Cd concentrations triggered a biphasic defence activation in the kidney that might lead to adaptation and survival. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
Notes: Hasselt Univ, Ctr Environm Sci, Lab Physiol, B-3590 Diepenbeek, Belgium. Hasselt Univ, Ctr Stat, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Ctr Environm Sci, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Univ Antwerp, Res Grp Plant Physiol, Dept Biol, B-2020 Antwerp, Belgium.Thijssen, S, Hasselt Univ, Ctr Environm Sci, Lab Physiol, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium.sandy_thijssen@hotmail.com emmy.vankerkhove@uhasselt.be
URI: http://hdl.handle.net/1942/4068
DOI: 10.1016/j.tox.2007.03.022
ISI #: 000247355200004
ISSN: 0300-483X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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