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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4064

Title: Functional analysis of D-alanylation of lipoteichoic acid in the probiotic strain Lactobacillus rhamnosus GG
Authors: Velez, Monica Perea
Verhoeven, Tine L. A.
Draing, Christian
Von Aulock, Sonja
Pfitzenmaier, Markus
Geyer, Armin
LAMBRICHTS, Ivo
Grangette, Corinne
Pot, Bruno
Vanderleyden, Jos
De Keersmaecker, Sigrid C. J.
Issue Date: 2007
Publisher: AMER SOC MICROBIOLOGY
Citation: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(11). p. 3595-3604
Abstract: Lipoteichoic acid (LTA) is a macroamphiphile molecule which performs several functions in gram-positive bacteria, such as maintenance of cell wall homeostasis. D-Alanylation of LTA requires the proteins encoded by the dlt operon, and this process is directly related to the charge properties of this polymer strongly contributing to its function. The insertional inactivation of dltD of the probiotic strain Lactobacillus rhamnosus GG (ATCC 53103) resulted in the complete absence Of D-alanyl esters in the LTA as confirmed by nuclear magnetic resonance analysis. This was reflected in modifications of the bacterial cell surface properties. The dltD strain showed 2.4-fold-increased cell length, a low survival capacity in response to gastric juice challenge, an increased sensitivity to human beta-defensin-2, an increased rate of autolysis, an increased capacity to initiate growth in the presence of an anionic detergent, and a decreased capacity to initiate growth in the presence of cationic peptides compared to wild-type results. However, in vitro experiments revealed no major differences for adhesion to human intestinal epithelial cells, biofilm formation, and immunomodulation. These properties are considered to be important for probiotics. The role of the dlt operon in lactobacilli is discussed in view of these results.
Notes: Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Louvain, Belgium. Univ Konstanz, Dept Biochem & Pharmacol, D-78457 Constance, Germany. Univ Marburg, Inst Organ Chem, D-35043 Marburg, Germany. Univ Hasselt, BIOMED Res Inst, Histol Lab, B-3590 Diepenbeek, Belgium. Inst Pasteur, F-59019 Lille, France.De Keersmaecker, SCJ, Katholieke Univ Leuven, Ctr Microbial & Plant Genet, Kasteelpk Arenberg 20, B-3001 Louvain, Belgium.sigrid.dekeersmaecker@biw.kuleuven.be
URI: http://hdl.handle.net/1942/4064
DOI: 10.1128/AEM.02083-06
ISI #: 000247016600018
ISSN: 0099-2240
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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