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Title: Detection of salivary alpha-amylase and lysozyme exposed on the pellicle formed in situ on different materials
Authors: Hannig, Christian
Huber, Karin
Graeser, Jan
Hannig, Matthias
Issue Date: 2007
Publisher: WILEY-LISS
Abstract: Amylase and lysozyme are components of the salivary pellicle, exposing considerable enzymatic activity in the immobilized state. The purpose of the present study was to elucidate the influence of different solid substrata on the amount and distribution of amylase and lysozyme exposed on the surface of the salivary pellicle formed in situ. Slabs of titanium, feldspar ceramic, and bovine enamel were fixed on the buccal sites of individual splints worn by three subjects for 3 or 30 min, respectively, to allow pellicle formation. Subsequently, slabs were removed from the splints and rinsed with running water. Detection of amylase and lysozyme was performed by FEI-SEM after gold-inummolabeling of the enzymes. Both enzymes were found to be distributed randomly at the pellicle surface. Irrespective of formation time and substratum, significantly more labeled lysozyme molecules (5.23 +/- 4.5 mu m(-2)) were detected compared with amylase (3.4 +/- 2.9 mu m(-2)). Neither the substratum nor the pellicle formation time had significant impact on the amount of the respective enzyme that could be detected. This study for the first time provides evidence, that amylase and lysozyme are exposed at the surface of the salivary pellicle formed in situ on titanium and ceramics. Both enzymes are distributed randomly on the surface of the pellicle, irrespective of the underlying substratum. (c) 2007 Wiley Periodicals.
Notes: Univ Freiburg, Dept Operat Dent & Periodontol, D-79106 Freiburg, Germany. Univ Saarland, Univ Hosp, Clin Operat Dent, D-66421 Homburg, Germany. Univ Hasselt, B-3590 Diepenbeek, Belgium. Scanning Elect Microscopy, Applicat Serv, Fresenius Med Care Deutschland GmbH, D-66606 St Wendel, Germany. Univ Hasselt, Mat Res Inst, B-3590 Diepenbeek, Belgium.Hannig, C, Univ Freiburg, Dept Operat Dent & Periodontol, Hugstetter Str 55, D-79106 Freiburg, Germany.christian.hannig@uniklinik-freiburg.de
URI: http://hdl.handle.net/1942/4061
DOI: 10.1002/jbm.a.31313
ISI #: 000249387300012
ISSN: 1549-3296
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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