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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4059

Title: An improved sample preparation for an LC method used in the age estimation based on aspartic acid racemization from human dentin
Authors: Yekkala, Raja
Meers, Carine
Hoogmartens, Jos
LAMBRICHTS, Ivo
WILLEMS, Guy
Van Schepdael, Ann
Issue Date: 2007
Publisher: WILEY-V C H VERLAG GMBH
Citation: JOURNAL OF SEPARATION SCIENCE, 30(1). p. 118-121
Abstract: The determination of age on the basis of aspartic acid (Asp) racemization in teeth is one of the most reliable and accurate methods to date. In this paper, the usefulness of HPLC coupled with fluorescence detection for determination of Asp racemization was evaluated. A modified sample preparation is proposed for better stability of o-phthaldialdehyde-N-acetyl-L-cysteine derivatives of D/L-Asp (due to the instability below pH 7). To ensure the accuracy of the method, the validation parameters' specificity, precision, linearity, and LOD were determined. Three dentin samples of premolar teeth, extracted from living individuals (bucco-lingual longitudinal sections of 1 mm thickness), were analyzed and quantitative results are discussed.
Notes: Katholieke Univ Leuven, Lab Pharmaceut Anal, B-3000 Louvain, Belgium. Katholieke Univ Leuven, Dept Forens Odontol, Sch Dent Oral Pathol & Maxillofacial Surg, Louvain, Belgium. Univ Hasselt, Lab Histol, Biomed Res Inst, Diepenbeek, Belgium.Van Schepdael, A, Katholieke Univ Leuven, Lab Pharmaceut Chem & Drug Anal, O&N 2,Postbus 923,Herestr 49, B-3000 Louvain, Belgium.ann.vanschepdael@pharm.kuleuven.be
URI: http://hdl.handle.net/1942/4059
DOI: 10.1002/jssc.200600191
ISI #: 000243776600018
ISSN: 1615-9306
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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