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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3911

Title: EFFECTS OF THE INTRODUCTION OF AGROBACTERIUM-TUMEFACIENS T-DNA IPT GENE IN NICOTIANA-TABACUM-L CV PETIT HAVANA SR1 PLANT-CELLS
Authors: Beinsberger, S.E.I.
VALCKE, Roland
DEBLAERE, RY
CLIJSTERS, Herman
DEGREEF, JA
VANONCKELEN, HA
Issue Date: 1991
Publisher: JAPANESE SOC PLANT PHYSIOLOGISTS
Citation: PLANT AND CELL PHYSIOLOGY, 32(4). p. 489-496
Abstract: Transformation of tobacco leaf discs with the 'cytokinin' ipt gene yielded several transgenic callus tissue lines, respective to the kind of ipt construction present in the A. tumefaciens cointegrates. Those calli containing an active ipt gene were able to grow hormone-autotrophically and showed an increased endogenous cytokinin level in comparison with controls. Analysis of endogenous IAA level did not allow any quantitative correlation with the cytokinin content. However, a minimal level of auxin seems to be necessary to obtain hormone-autotrophic growth. Exogenously supplied NAA significantly reduced the endogenous cytokinin content without modifying growth characteristics. The varying chlorophyll content in the different callus lines elicited the study of the ultrastructure of the plastids. The controls contained small plastids, often filled with starch or accumulated vesicles that did not allow observation of the internal membrane system. The 'Pssu-ipt' line, having a higher cytokinin content, showed plastids with an internal membrane system consisting of stroma and grana thylakoids, but this structure was lost during subculture. Swollen thylakoids appeared, the amount of starch was reduced and vesicles were accumulating.
Notes: LIMBURGS UNIV CENTRUM,DEPT SBM,B-3590 DIEPENBEEK,BELGIUM. STATE UNIV GHENT,GENET LAB,B-9000 GHENT,BELGIUM.BEINSBERGER, SEI, UNIV INSTELLING ANTWERP,DEPT BIOL,B-2610 WILRIJK,BELGIUM.
URI: http://hdl.handle.net/1942/3911
Link to publication: http://pcp.oxfordjournals.org/cgi/content/abstract/32/4/489
ISI #: A1991FR38400005
ISSN: 0032-0781
Type: Journal Contribution
Appears in Collections: Research publications

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