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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3895

Title: BASEMENT-MEMBRANE COMPONENTS (MATRIGEL) PROMOTE THE TUMORIGENICITY OF HUMAN BREAST ADENOCARCINOMA MCF7 CELLS AND PROVIDE AN INVIVO MODEL TO ASSESS THE RESPONSIVENESS OF CELLS TO ESTROGEN
Authors: NOEL, A
Simon, N.
RAUS, Jef
FOIDART, JM
Issue Date: 1992
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: BIOCHEMICAL PHARMACOLOGY, 43(6). p. 1263-1267
Abstract: The ability to transplant human tumors into athymic nude mice allows studies of tumor cells in vivo. However, after s.c. injection the incidence of tumor and metastases in nude mice is frequently low. We have studied the tumorigenicity in nude mice of estradiol (E2)-sensitive breast adenocarcinoma MCF7 cells. Matrigel, an extract of basement membrane proteins, induces rapid tumor development after s.c. injection of MCF7 cells. In the absence of this matrice, MCF7 cells failed to induce tumor growth. In this in vivo model, MCF7 cells were analysed for their E2 sensitivity. Two weeks after inoculation in the presence of matrigel, cells formed growing tumors in intact mice supplemented with E2. In ovariectomized or untreated mice, tumor appearance was delayed and the growth level was very low. Thus, MCF7 cells formed tumors in the absence of E2 but retained in vivo their responsiveness to estrogen. Growing human tumors in nude mice provides a rapid and useful model for testing the sensitivity of cells to hormone.
Notes: UNIV LIEGE BIOL LAB,LIEGE,BELGIUM. DR L WILLEMS INST,DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3895
DOI: 10.1016/0006-2952(92)90501-9
ISI #: A1992HM25600013
ISSN: 0006-2952
Type: Journal Contribution
Appears in Collections: Non-affiliated authors

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