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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3885

Title: MYELIN BASIC-PROTEIN SPECIFIC LYMPHOCYTE-T IN MULTIPLE-SCLEROSIS AND CONTROLS - PRECURSOR FREQUENCY, FINE SPECIFICITY, AND CYTOTOXICITY
Authors: HASHIM, GA
YING, C
VANDENBERG-LOONEN, E
MEDAER, Rob
RAUS, Jef
Zhang, JK
Issue Date: 1992
Publisher: LITTLE BROWN CO
Citation: ANNALS OF NEUROLOGY, 32(3). p. 330-338
Abstract: A panel of 90 myelin basic protein (MBP)-specific T-cell lines were derived from peripheral blood of eight with multiple sclerosis and four normal subjects. The precursor frequency of MBP-reactive T cells in peripheral blood mononuclear cells ranged from 10(-7) to 9 X 10(-7) (mean, 6.7 x 10(-7)) in the group of patients with multiple sclerosis and from 0.5 x 10(-7) to 9.8 X 10(-7) (mean, 5.6 x 10(-7)) in the control subjects. This difference between the two groups was not statistically significant (p > 0.1). These T-cell lines expressed exclusively CD3+CD4+CD8- phenotypes and were restricted predominantly by HLA-DR molecules. When tested with fragments and synthetic peptides of human MBP, these MBP-specific T-cell lines (45 lines for each group) displayed a limited heterogeneous pattern with a biased recognition to peptide 84-102 and the C-terminal peptide 149-171. The reactivity to the 84-102 region of MBP was associated with the HLA-DR2, DRw15 (DRw15,2) haplotype, whereas the recognition to peptide 149-171 did not correlate with a particular HLA-DR allele(s). Furthermore, the majority of T-cell lines (> 75%) were found to exhibit substantial cytotoxic activity against MBP-coated target cells, but showing no significant difference between these two groups. This MBP-dependent cytotoxicity was not associated with epitope specificities of the T-cell lines tested.
Notes: MULTIPLE SCLEROSIS CLIN & REHABIL CTR,OVERPELT,BELGIUM. ST LUKES ROOSEVELT HOSP,NEW YORK,NY 10025. COLUMBIA UNIV,NEW YORK,NY 10027. ACAD HOSP MAASTRICHT,TISSUE TYPING LAB,MAASTRICHT,NETHERLANDS. LIMBURGS UNIV CENTRUM,B-3610 DIEPENBEEK,BELGIUM. DR L WILLEMS INST,MULTIPLE SCLEROSIS RES LAB,DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3885
ISI #: A1992JN14500004
ISSN: 0364-5134
Type: Journal Contribution
Appears in Collections: Research publications

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