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|Title: ||LARGE-SCALE EXPANSION OF HUMAN TUMOR INFILTRATING LYMPHOCYTES WITH SURFACE-MODIFIED STIMULATOR CELLS FOR ADOPTIVE IMMUNOTHERAPY|
|Authors: ||CHIN, Y|
|Issue Date: ||1992|
|Publisher: ||INT INST ANTICANCER RESEARCH|
|Citation: ||ANTICANCER RESEARCH, 12(3). p. 733-736|
|Abstract: ||Expansion of tumor infiltrating lymphocytes (TIL) in vitro is hampered by several factors, including a limited amount of lymphocytes obtained from different tumors, unknown target antigens and limited supply of antigen-presenting cells (APC) which are generally believed to be essential in the classical way of T cell stimulation and expansion. In approaching these difficulties, we have recently used surface-oxidized allogeneic PBL to stimulate the TIL periodically in the presence of a low dose of rIL-2 (200 IU/ml). TIL derived from 22 (out of 23) tumor specimens could be expanded with 20-10(7) fold increases over 6-16 weeks to a sufficient amount of 10(9)-10(11) cells for adoptive immunotherapy. In contrast, only 2-100 fold increase were observed in six tumor specimens (out of 23) when 200 IU/ml rIL-2 was used only. The phenotypes, autologous tumor reactivity and cytolytic capability of TIL propagated with surface-oxidized stimulators were similar to those expanded in the presence of IL-2 alone. These data suggest that expanding TIL with surface-modified stimulator cells could be a useful alternative method to obtain a large amount of tumor specific cytolytic T cells for clinical immunotherapeutic use, irrespective of tumor-antigen stimulation and MHC restriction.|
|Notes: ||DR L WILLEMS INST,DEPT CANC RES,B-3590 DIEPENBEEK,BELGIUM. LIMBURGS ONCOL CTR,B-3500 HASSELT,BELGIUM. LIMBURG STATE UNIV CTR,B-3590 DIEPENBEEK,BELGIUM.|
|ISI #: ||A1992JB31600022|
|Type: ||Journal Contribution|
|Appears in Collections: ||Research publications|
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