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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3856

Title: LARGE-SCALE EXPANSION OF HUMAN TUMOR INFILTRATING LYMPHOCYTES WITH SURFACE-MODIFIED STIMULATOR CELLS FOR ADOPTIVE IMMUNOTHERAPY
Authors: CHIN, Y
JANSSENS, Jaak
SMEYERS, Els
BLEUS, Josianne
RAUS, Jef
Zhang, JK
Issue Date: 1992
Publisher: INT INST ANTICANCER RESEARCH
Citation: ANTICANCER RESEARCH, 12(3). p. 733-736
Abstract: Expansion of tumor infiltrating lymphocytes (TIL) in vitro is hampered by several factors, including a limited amount of lymphocytes obtained from different tumors, unknown target antigens and limited supply of antigen-presenting cells (APC) which are generally believed to be essential in the classical way of T cell stimulation and expansion. In approaching these difficulties, we have recently used surface-oxidized allogeneic PBL to stimulate the TIL periodically in the presence of a low dose of rIL-2 (200 IU/ml). TIL derived from 22 (out of 23) tumor specimens could be expanded with 20-10(7) fold increases over 6-16 weeks to a sufficient amount of 10(9)-10(11) cells for adoptive immunotherapy. In contrast, only 2-100 fold increase were observed in six tumor specimens (out of 23) when 200 IU/ml rIL-2 was used only. The phenotypes, autologous tumor reactivity and cytolytic capability of TIL propagated with surface-oxidized stimulators were similar to those expanded in the presence of IL-2 alone. These data suggest that expanding TIL with surface-modified stimulator cells could be a useful alternative method to obtain a large amount of tumor specific cytolytic T cells for clinical immunotherapeutic use, irrespective of tumor-antigen stimulation and MHC restriction.
Notes: DR L WILLEMS INST,DEPT CANC RES,B-3590 DIEPENBEEK,BELGIUM. LIMBURGS ONCOL CTR,B-3500 HASSELT,BELGIUM. LIMBURG STATE UNIV CTR,B-3590 DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3856
ISI #: A1992JB31600022
ISSN: 0250-7005
Type: Journal Contribution
Appears in Collections: Research publications

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