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|Title: ||REGULATION OF MYELIN BASIC PROTEIN-SPECIFIC HELPER T-CELLS IN MULTIPLE-SCLEROSIS - GENERATION OF SUPPRESSOR T-CELL LINES|
|Authors: ||Zhang, JK|
|Issue Date: ||1992|
|Publisher: ||ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS|
|Citation: ||CELLULAR IMMUNOLOGY, 139(1). p. 118-130|
Suppressor T cell (Ts) lines specific for myelin basic protein (MBP)-reactive helper T cell (Th) clones were generated from two patients with multiple sclerosis (MS) following a primary culture of peripheral blood mononuclear cells (PBMC) with MBP and cyclosporine A (CsA). These suppressor T cell lines were maintained in culture by alternate stimulation with MBP and antigen-presenting cells (APC). The Ts lines expressed preferentially the CD4 phenotype (5/6 Ts lines tested) and exhibited potent antigen-specific suppressor activity on the proliferation of MBP-specific Th clones and not on the T cell lines with other antigen specificity. For some Ts lines, a Ts-to-Th ratio of 1 was sufficient to inhibit the proliferation of MBP-specific T cells by 90%. The suppressor T cells obtained were weakly responsive to MBP and required the presence of the autologous PBMC for proliferation. Furthermore, proliferation of these suppressor T cell lines was restricted by HLA-DR molecules (for CD4+ Ts lines) and HLA class I (for a CD8+ Ts line). The suppressor T cell lines generated and the techniques described in this study may be helpful in our understanding of the events involved in the immune regulation in MS and other autoimmune diseases.|
|Notes: ||DR L WILLEMS INST,DEPT IMMUNOL,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM. MULTIPLE SCLEROSIS CLIN & REHABIL CTR,OVERPELT,BELGIUM. LIMBURGS UNIV CENTRUM,B-3610 DIEPENBEEK,BELGIUM.|
|ISI #: ||A1992GW21300011|
|Type: ||Journal Contribution|
|Appears in Collections: ||Research publications|
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