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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3836

Title: CONSTRUCTION AND EXPRESSION OF ANTIBODY TUMOR-NECROSIS-FACTOR FUSION PROTEINS
Authors: HOOGENBOOM, HR
VOLCKAERT, G
RAUS, Jef
Issue Date: 1991
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: MOLECULAR IMMUNOLOGY, 28(9). p. 1027-1037
Abstract: The construction, expression and secretion of two genetically engineered antibody-cytokine hybrid fusion proteins is described. To target tumor necrosis factor (TNF) to tumor cells, recombinant antibody techniques were used to generate F(ab')2-like antibody-TNF fusion proteins. At the gene level, an antitransferrin receptor antibody heavy chain gene was linked to a synthetic gene coding for human TNF. The chimeric heavy chain-TNF genes were introduced into a light chain secreting transfectoma cell line, which was producing the light chain of the same antibody. Cell lines were isolated which secreted antibody-TNF fusion proteins of expected size and composition. Culture supernatant of these cell lines contained TNF cytotoxic activity towards murine L929 cells and human MCF-7 cells, indicating that TNF is still active in the fusion protein constructs. These results illustrate the feasibility of the antibody engineering technology to create and produce chimeric mouse-human immunotoxin-like molecules. Furthermore, they demonstrate the ability of mammalian (myeloma) cells to express and secrete antibody-cytokine hybrid molecules with potential use in anticancer therapy.
Notes: LIMBURGS UNIV CENTRUM,DR L WILLEMS INST,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM. LIMBURGS UNIV CENTRUM,DEPT WNIF,B-3590 DIEPENBEEK,BELGIUM. CATHOLIC UNIV LEUVEN,GENTECHNOL LAB,B-3030 HEVERLEE,BELGIUM.
URI: http://hdl.handle.net/1942/3836
DOI: 10.1016/0161-5890(91)90189-Q
ISI #: A1991GJ82700013
ISSN: 0161-5890
Type: Journal Contribution
Appears in Collections: Research publications

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