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|Title: ||CONSTRUCTION AND EXPRESSION OF ANTIBODY TUMOR-NECROSIS-FACTOR FUSION PROTEINS|
|Authors: ||HOOGENBOOM, HR|
|Issue Date: ||1991|
|Publisher: ||PERGAMON-ELSEVIER SCIENCE LTD|
|Citation: ||MOLECULAR IMMUNOLOGY, 28(9). p. 1027-1037|
|Abstract: ||The construction, expression and secretion of two genetically engineered antibody-cytokine hybrid fusion proteins is described. To target tumor necrosis factor (TNF) to tumor cells, recombinant antibody techniques were used to generate F(ab')2-like antibody-TNF fusion proteins. At the gene level, an antitransferrin receptor antibody heavy chain gene was linked to a synthetic gene coding for human TNF. The chimeric heavy chain-TNF genes were introduced into a light chain secreting transfectoma cell line, which was producing the light chain of the same antibody. Cell lines were isolated which secreted antibody-TNF fusion proteins of expected size and composition. Culture supernatant of these cell lines contained TNF cytotoxic activity towards murine L929 cells and human MCF-7 cells, indicating that TNF is still active in the fusion protein constructs. These results illustrate the feasibility of the antibody engineering technology to create and produce chimeric mouse-human immunotoxin-like molecules. Furthermore, they demonstrate the ability of mammalian (myeloma) cells to express and secrete antibody-cytokine hybrid molecules with potential use in anticancer therapy.|
|Notes: ||LIMBURGS UNIV CENTRUM,DR L WILLEMS INST,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM. LIMBURGS UNIV CENTRUM,DEPT WNIF,B-3590 DIEPENBEEK,BELGIUM. CATHOLIC UNIV LEUVEN,GENTECHNOL LAB,B-3030 HEVERLEE,BELGIUM.|
|ISI #: ||A1991GJ82700013|
|Type: ||Journal Contribution|
|Appears in Collections: ||Research publications|
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