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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3767

Title: DEVELOPMENT AND FUNCTIONAL-CHARACTERIZATION OF A MURINE HUMAN CHIMERIC ANTIBODY WITH SPECIFICITY FOR THE HUMAN INTERLEUKIN-2 RECEPTOR
Authors: VANDEVYVER, Caroline
STEUKERS, M
LAMBRECHT, Johan
HEYLIGEN, Harie
RAUS, Jef
Issue Date: 1993
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: MOLECULAR IMMUNOLOGY, 30(10). p. 865-876
Abstract: A murine/human chimeric antibody, with specificity for the human interleukin-2 receptor, was developed by genetic engineering. For this purpose, the light and heavy chain variable region exons encoding the murine monoclonal antibody 2C8 were isolated and inserted into expression vectors containing the human kappa and gamma-1 constant regions. After transfection by electroporation of the chimeric genes into murine Sp 2/0 hybridoma cells, transfectomas secreting the complete chimeric antibody were selected. The chimeric antibody has similar binding properties as the original murine antibody. The in vitro cytotoxic effects of the murine and the chimeric antibodies were compared.
Notes: LIMBURG UNIV CENTRUM,B-3590 DIEPENBEEK,BELGIUM.VANDEVYVER, C, DR L WILLEMS INST,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3767
ISI #: A1993LP87800002
ISSN: 0161-5890
Type: Journal Contribution
Appears in Collections: Non-affiliated authors

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