Document Server@UHasselt >
Research publications >
Please use this identifier to cite or link to this item:
|Title: ||CELLULAR AND MOLECULAR ANALYSIS OF TUMOR-INFILTRATING T-LYMPHOCYTES FROM HUMAN BREAST-CANCER AT CLONAL LEVEL|
|Authors: ||CHIN, Y|
|Issue Date: ||1994|
|Publisher: ||INT JOURNAL ONCOLOGY|
|Citation: ||ONCOLOGY REPORTS, 1(3). p. 577-583|
|Abstract: ||51 T cell lines/clones were established from tumor-infiltrating lymphocytes of nine breast tumors by limiting dilution. All the lines/clones were exclusively CD3+ and expressed either CD4 (57%) or CD8 (26%) phenotype. In addition, 17% of the lines/clones displayed a dual expression of CD4+CD8+ antigens. No CD3-CD16+ NK clones were obtained. A vast majority of the T cell lines and clones (84%) exhibited cytolytic activity in a lectin-dependent assay which allows the detection of cytolytic T cells of any antigen specificity. 17% of the lines/clones lysed two allogeneic breast tumor cell lines, MCF-7 and HBL-100. 11% of the cells showed NK-like cytolysis by lysing an NK-sensitive cell line K562. Of the 17 lines tested against autologous tumor cells, only two exhibited cytolytic activity via T cell receptor and CD3 molecule in an MHC-restricted manner. Southern blot analysis of T cell receptor of 39 lines/clones revealed a limited heterogeneity of TCR-B chain gene rearrangements, which suggests oligoclonal expansion of T cells infiltrating into the tumor.|
|Notes: ||LIMBURGS UNIV CENTRUM,B-3610 DIEPENBEEK,BELGIUM.CHIN, Y, DR L WILLEMS INST,DEPT CANC RES,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM.|
|ISI #: ||A1994PW22400022|
|Type: ||Journal Contribution|
|Appears in Collections: ||Research publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.