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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3714

Title: MATRIX EFFECTS IN THE RADIOIMMUNOASSAY OF ESTRADIOL AND TESTOSTERONE IN PLASMA OF VEAL CALVES AND HOW TO AVOID THEM
Authors: Pottie, G.
Bagyary, S.
Hendriks, Lutgardis
Gielen, Brigitte
Haesen, Veronique
Raus, Jef
Issue Date: 1993
Publisher: ELSEVIER SCIENCE BV
Citation: ANALYTICA CHIMICA ACTA, 275(1-2). p. 113-122
Abstract: Within the scope of the control of the illegal use of naturally occurring steroids in veal calves, an evaluation was made of two commercially available kits for the determination of estradiol-17beta and testosterone in plasma. Four radioimmunoassay (RIA) kits, two with and two without extraction, used in human medicine were evaluated and eventually adapted for the assay of bovine plasma samples. On measuring estradiol-170 lower values were obtained when the plasma samples were assayed without extraction (I-125-RIA). This difference was due to a plasma matrix effect which could be avoided by using either the extraction method (H-3-RIA) or by dissolving estradiol standards in bovine plasma instead of human serum. This plasma matrix effect did not interfere as much when testosterone levels were measured. However, the best method for the determination of both steroids in bovine plasma was RIA with extraction (H-3-RIA).
Notes: DR L WILLEMS INST,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM. INST HYG & EPIDEMIOL,B-1050 BRUSSELS,BELGIUM. LIMBURGS UNIV CENTRUM,B-3590 DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3714
ISI #: A1993KV27900013
ISSN: 0003-2670
Type: Journal Contribution
Appears in Collections: Research publications

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