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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3439

Title: Regulation of proliferation of LLC-MK(2) cells by nucleosides and nucleotides: The role of ecto-enzymes
Authors: LEMMENS, Raf
VANDUFFEL, Luc
TEUCHY, Henri
Culic, O
Issue Date: 1996
Publisher: PORTLAND PRESS
Citation: BIOCHEMICAL JOURNAL, 316. p. 551-557
Abstract: 1. Using the incorporation of [methyl-H-3]thymidine as a proliferation marker, the effects of various nucleosides and nucleotides on endothelial LLC-MK(2) cells were studied. We found that ATP, ADP, AMP and adenosine in concentrations of 10 mu M or higher stimulate the proliferation of these cells. 2. Inhibition of ecto-ATPase (EC 3.6.1.15), 5'-nucleotidase (EC 3.1.3.5) or alkaline phosphatase (EC 3.1.3.1) significantly diminished the stimulatory effect of ATP, indicating that the effect is primarily caused by adenosine and not by adenine nucleotides. Also, the effect depends only on extracellular nucleosides, since inhibition of nucleoside uptake by dipyridamole has no influence on proliferation. 3. Other purine nucleotides and nucleosides (ITP, GTP, inosine and guanosine) also stimulate cell proliferation, while pyrimidine nucleotides and nucleosides (CTP, UTP, cytidine and uridine) inhibit proliferation. Furthermore, the simultaneous presence of adenosine and any of the other purine nucleosides is not entirely additive in its effect on cell proliferation. At the same time any pyrimidine nucleoside, when added together with adenosine, has the same inhibitory effect as the pyrimidine nucleoside alone. 4. Apparently these proliferative effects are neither caused by any pharmacologically known P-1-purinoceptor, nor are they mediated by cyclic AMP, cyclic GMP, or D-myo-inositol 1,4,5-trisphosphate as second messenger, nor by extracellular Ca2+. 5. Therefore, we conclude that various purine and pyrimidine nucleosides can influence the proliferation of LLC-MK(2) cells by acting on putative purinergic and pyrimidinergic receptors not previously described.
Notes: Lemmens, R, LIMBURGS UNIV CENTRUM,DEPT MBW,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3439
Link to publication: http://www.biochemj.org/bj/316/bj3160551.htm
ISI #: A1996UT23200028
ISSN: 0264-6021
Type: Journal Contribution
Appears in Collections: Research publications

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