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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3418

Title: Copper toxicity in young maize (Zea mays L) plants: Effects on growth, mineral and chlorophyll contents, and enzyme activities
Authors: Mocquot, Bernard
Vangronsveld, Jaco
Clijsters, Herman
Mench, Michel
Issue Date: 1996
Publisher: KLUWER ACADEMIC PUBL
Citation: PLANT AND SOIL, 182(2). p. 287-300
Abstract: Changes in the growth parameters and in enzyme activities were studied in roots and leaves of 14-days old maize grown in a nutrient solution containing various copper concentrations (i.e. 0.01 to 10 mu M). A significant decrease in root and leaf biomass was only found at 10 mu M Cu. In contrast, changes in several enzyme activities occured at lower copper concentrations in the solution, corresponding to different threshold values which are lower than those observed for growth parameters. Peroxidase (POD) activity significantly increased in all investigated plant organs (i.e. 3rd-leaf, 4th-leaf and roots) in relation to their copper content. Additionally, glucose-6-phosphate dehydrogenase (G-6-PDH), and isocitrate dehydrogenase (ICDH) activities decreased in the leaves, especially in the 4th-leaf. However, the activity of malic enzyme (ME), G-6-PDH, glutamate dehydrogenase (GDH) and ICDH increased with the copper content in roots. According to the relationship between POD activity and copper content, the toxic critical value was set at 26 mg Cu per kg dry matter (DM) in roots and 21 mg Cu per kg DM in the 3rd-leaf. In roots, a new isoenzyme of peroxidase appeared for copper content above 12.6 mg Cu kg DM(-1). Measurement of enzyme activity, especially that of POD and Cu-specific changes in the (iso)peroxidase pattern, might be used as biomarkers to assess the phytotoxicity for maize grown on copper-contaminated substrata.
Notes: LIMBURGS UNIV CTR,DEPT SBG,B-3590 DIEPENBEEK,BELGIUM.Mocquot, B, INRA,BORDEAUX AQUITAINE RES CTR,AGRON UNIT,BP 81,F-33883 VILLENAVE DORNON,FRANCE.
URI: http://hdl.handle.net/1942/3418
DOI: 10.1007/BF00029060
ISI #: A1996VW63100012
ISSN: 0032-079X
Type: Journal Contribution
Appears in Collections: Research publications

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