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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3416

Title: Immunotherapy for cancer: Construction, expression and functional characterization of chimeric antibodies
Authors: Motmans, K
Thirion, S
VANDEVYVER, Caroline
HEYLIGEN, Harie
JANSSENS, Jaak
RAUS, Jef
Issue Date: 1996
Publisher: RAPID SCIENCE PUBLISHERS
Citation: EUROPEAN JOURNAL OF CANCER PREVENTION, 5(6). p. 512-519
Abstract: Monoclonal antibodies (Mabs) are a potential key component for the treatment of cancer, because of their specificity and multiple effector functions, Hybridoma technology and progress in genetic engineering made it possible to customize antibody molecules, rendering them more suitable for selective application, A widely used technique is the construction of mouse-human hybrid molecules by recombinant DNA techniques, These so-called chimeric antibodies contain the murine variable (V) regions fused to the human constant (C) regions, In this report, a general approach is described for the production of chimeric antibodies, The gene segments encoding the murine variable heavy and light chain are isolated by the polymerase chain reaction and cloned into expression vectors containing the human yl heavy chain gene and the human K light chain gene, respectively, Subsequently, these constructs are transfected into a non-Ig-producing murine hybridoma, eg SP2/0 cells, The in vitro study of the functional characteristics and biological properties of the thus obtained chimeric antibodies are discussed.
Notes: DR WILLEMS INST,DEPT BIOTECHNOL,B-3590 DIEPENBEEK,BELGIUM. LIMBURGS UNIV CTR,B-3590 DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3416
ISI #: A1996WL97600015
ISSN: 0959-8278
Type: Journal Contribution
Appears in Collections: Research publications

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