Document Server@UHasselt >
Research publications >
Please use this identifier to cite or link to this item:
|Title: ||Potential misevaluation of the ground-state dissociation constant from fluorimetric titrations: Application to the ion indicators SBFI, PBFI, and Fura-2|
|Authors: ||Kowalczyk, A|
|Issue Date: ||1997|
|Publisher: ||ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS|
|Citation: ||ANALYTICAL BIOCHEMISTRY, 245(1). p. 28-37|
|Abstract: ||A test based on time-resolved fluorescence experiments is proposed to assess the interference of an excited-state reaction with the fluorimetric determination of the ground-state dissociation constant K-d of ion subset of fluorescent indicator complexes. If an inflection point occurs in the plot of the fluorescence signal vs -log [ion] in the concentration range of the ion where both decay times are invariant, this inflection point can be associated With the correct K-d. In contrast, the inflection point(s) in the concentration range where the decay times vary cannot be attributed to K-d. The test is applied to the fluorescent ion indicators SBFI (for Na+), PBFI (for K+), and Fura-2 (for Ca2+). In all three cases the decay times are invariant in the physiological concentration ranges of the respective ions, indicating that the fluorimetrically determined K-d values are actually the true K-d values. (C) 1997 Academic Press.|
|Notes: ||NICHOLAS COPERNICUS UNIV,INST PHYS,PL-87100 TORUN,POLAND. KATHOLIEKE UNIV LEUVEN,DEPT CHEM,B-3001 HEVERLEE,BELGIUM. LIMBURGS UNIV CTR,B-3590 DIEPENBEEK,BELGIUM.|
|ISI #: ||A1997WE92400004|
|Type: ||Journal Contribution|
|Appears in Collections: ||Research publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.