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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3308

Title: Potential misevaluation of the ground-state dissociation constant from fluorimetric titrations: Application to the ion indicators SBFI, PBFI, and Fura-2
Authors: Kowalczyk, A
Boens, N
Meuwis, K
AMELOOT, Marcel
Issue Date: 1997
Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Citation: ANALYTICAL BIOCHEMISTRY, 245(1). p. 28-37
Abstract: A test based on time-resolved fluorescence experiments is proposed to assess the interference of an excited-state reaction with the fluorimetric determination of the ground-state dissociation constant K-d of ion subset of fluorescent indicator complexes. If an inflection point occurs in the plot of the fluorescence signal vs -log [ion] in the concentration range of the ion where both decay times are invariant, this inflection point can be associated With the correct K-d. In contrast, the inflection point(s) in the concentration range where the decay times vary cannot be attributed to K-d. The test is applied to the fluorescent ion indicators SBFI (for Na+), PBFI (for K+), and Fura-2 (for Ca2+). In all three cases the decay times are invariant in the physiological concentration ranges of the respective ions, indicating that the fluorimetrically determined K-d values are actually the true K-d values. (C) 1997 Academic Press.
Notes: NICHOLAS COPERNICUS UNIV,INST PHYS,PL-87100 TORUN,POLAND. KATHOLIEKE UNIV LEUVEN,DEPT CHEM,B-3001 HEVERLEE,BELGIUM. LIMBURGS UNIV CTR,B-3590 DIEPENBEEK,BELGIUM.
URI: http://hdl.handle.net/1942/3308
DOI: 10.1006/abio.1996.9932
ISI #: A1997WE92400004
ISSN: 0003-2697
Type: Journal Contribution
Appears in Collections: Research publications

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