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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3209

Title: Identification of some important metabolites of boldenone in urine and feces of cattle by gas chromatography mass spectrometry
Authors: VAN PUYMBROECK, Mark
Kuilman, MEM
Maas, RFM
Witkamp, RF
LEYSSENS, Luc
VANDERZANDE, Dirk
GELAN, Jan
RAUS, Jef
Issue Date: 1998
Publisher: ROYAL SOC CHEMISTRY
Citation: ANALYST, 123(12). p. 2681-2686
Abstract: 17 alpha-Boldenone (17 alpha-BOL) and/or 17 beta-boldenone (17 beta-BOL) appear occasionally in fecal matter of cattle. In addition to 17 alpha-BOL, a whole array of boldenone related substances can be found in the same samples. In vitro experiments with microsomal liver preparations and isolated hepatocytes combined with the excretion profiles found in urine and feces samples of in vivo experiments made it possible to identify several metabolites of 17 beta-BOL in 17 beta-BOL positive feces samples. In one animal treated with 17 beta-BOL, no 17 beta-BOL or its metabolites were present before treatment and most of these compounds disappeared,gradually in time after the treatment was stopped. It is not clear what the origin is of 17 alpha-BOL and boldenone metabolites in samples screened routinely for the abuse of anabolic steroids and considered to be 'negative' because of the absence of 17 beta-BOL since other workers showed some evidence that 17 alpha-BOL can be of endogenous origin. However, in our hands, most of these 17 alpha-BOL positive samples, obtained during routinely performed screenings of cattle, contained large amounts of Delta(4)-androstene-3,17-dione (PLED), which normally is absent from routinely screened negative samples. Furthermore, AED was absent in all samples obtained from the animals treated with 17 beta-BOL. We have no direct evidence that 17 alpha-BOL or 17 beta-BOL is of endogenous origin.
Notes: Dr L Willems Inst, Dept Drug & Residue Anal, B-3590 Diepenbeek, Belgium. Limburgs Univ Ctr, Dept SBM, B-3590 Diepenbeek, Belgium. Univ Utrecht, Dept Vet Pharmacol Pharm & Toxicol, NL-3508 TD Utrecht, Netherlands. TNO Pharma, NL-3700 AJ Zeist, Netherlands.Van Puymbroeck, M, Dr L Willems Inst, Dept Drug & Residue Anal, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/3209
ISI #: 000077607100058
ISSN: 0003-2654
Type: Journal Contribution
Validation: ecoom, 2000
Appears in Collections: Research publications

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