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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3202

Title: Photophysics of SBFO: A fluorescent indicator for Na+
Authors: Meuwis, K
Boens, N
De Schryver, FC
AMELOOT, Marcel
Gallay, J
Vincent, M
Issue Date: 1998
Publisher: AMER CHEMICAL SOC
Citation: JOURNAL OF PHYSICAL CHEMISTRY B, 102(3). p. 641-648
Abstract: SBFO is a commercially available fluorescent indicator for the determination of Na+ concentrations. To investigate the reversible reaction of Na+ with this indicator in the ground and excited states, steady-state and time-resolved fluorescence measurements were performed. Global compartmental analysis was used to describe the fluorescence decay surfaces yielding the following values for the rate constants at room temperature in aqueous solution at pH 7.2: k(01) = 5 x 10(8) s(-1), 2 x 10(7) M-1 s(-1) < k(21) < 3 x 10(8) M-1 s(-1), k(02) = 5 x 10(8) s(-1), k(12) = 1 x 10(8) s(-1). k(01) and k(02) denote the respective deactivation rate constants of the Na+ free and bound forms of the indicator in the excited state. k(21) represents the second-order rate constant of association of Na+ with SBFO in the excited state, whereas k(12) is the first-order rate constant of dissociation of the excited Na+-indicator complex. The effect of the reversible excited-state reaction between Na+ and the indicator on the fluorimetric determination of K-d can be neglected.
Notes: Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium. Limburgs Univ Ctr, B-3590 Diepenbeek, Belgium. Ctr Univ Paris Sud, Utilisat Rayonnement Electromagnet Lab, F-91405 Orsay, France.Boens, N, Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium.Noel.Boens@chem.kuleuven.ac.be
URI: http://hdl.handle.net/1942/3202
ISI #: 000071802700023
ISSN: 1089-5647
Type: Journal Contribution
Validation: ecoom, 1999
Appears in Collections: Research publications

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