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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3192

Title: Cadmium toxicity effects on growth, mineral and chlorophyll contents, and activities of stress related enzymes in young maize plants (Zea mays L.)
Authors: Lagriffoul, Arnoud
Mocquot, Bernard
Mench, Michel
Vangronsveld, Jaco
Issue Date: 1998
Publisher: KLUWER ACADEMIC PUBL
Citation: PLANT AND SOIL, 200(2). p. 241-250
Abstract: Plants were cultivated in a nutrient solution containing increasing cadmium concentrations (i.e. 0.001-25 mu M), under strictly controlled growth conditions. Changes in both growth parameters and enzyme activities, directly or indirectly related to the cellular free radical scavenging systems, were studied in roots and leaves of 14-day-old maize plants (Zea mays L., cv. Volga) as a result of Cd uptake. A decrease in both shoot length and leaf dry biomass was found to be significant only when growing on 25 mu M Cd, whereas concentrations of chlorophyll pigments in the 4th leaf decreased from 1.7 mu M Cd on. Changes in enzyme activities occurred at lower Cd concentrations in solution leading to lower threshold values for Cd contents in plants than those observed for growth parameters. Peroxidase (POD; E.C. 1.11.1.7)activity increased in the 3rd and 4th leaf, but not in roots. In contrast, glucose 6-phosphate dehydrogenase (G6PDH; E.C. 1.1.1.49), isocitrate dehydrogenase (ICDH; E.C. 1.1.1.42) and malic enzyme (ME; E.C. 1.1.1.40) activities decreased in the 3rd leaf. According to the relationship between the POD activity and the Cd content, a toxic critical value was set at 3 mg Cd per kg dry matter in the 3rd leaf and 5 mg Cd per kg dry matter in the 4th. Anionic POD were determined both in root and leaf protein extracts; however, no changes in the isoperoxidase pattern were detected in case of Cd toxicity. Results show that in contrast with growth parameters, the measurement of enzyme activities may be included as early biomarkers in a plant bioassay to assess the phytotoxicity of Cd-contaminated soils on maize plants.
Notes: INRA, Bordeaux Aquitaine Res Ctr, Agron Unit, F-33883 Villenave Dornon, France. Limburgs Univ Ctr, Dept SBG, B-3590 Diepenbeek, Belgium.Lagriffoul, A, INRA, Bordeaux Aquitaine Res Ctr, Agron Unit, BP 81, F-33883 Villenave Dornon, France.Lagriffo@bordeaux.inra.fr
URI: http://hdl.handle.net/1942/3192
Link to publication: https://www.researchgate.net/publication/226857980_Cadmium_toxicity_effects_on_growth_mineral_and_chlorophyll_contents_and_activities_of_stress_related_enzymes_in_young_maize_plants_Zea_mays_L
DOI: 10.1023/A:1004346905592
ISI #: 000074849600013
ISSN: 0032-079X
Type: Journal Contribution
Validation: ecoom, 1999
Appears in Collections: Research publications

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